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Decreased lung carcinoma cell density on select polymer nanometer surface features for lung replacement therapies

Published online by Cambridge University Press:  31 January 2011

Lijuan Zhang ,
Young Wook chun  and
Thomas J Webster
Lijuan Zhang
Affiliation:
[email protected], Brown University, chemistry, Providence, Rhode Island, United States
Young Wook chun
Affiliation:
[email protected], Brown University, engineering, providence, Rhode Island, United States
Thomas J Webster
Affiliation:
[email protected], Brown University, engineering, providence, Rhode Island, United States
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Abstract

PLGA (poly-lactic-co-glycolic acid) has been widely used as a biomaterial in regenerative medicine due to its biocompatibility and biodegradability properties. Previous studies have shown that cells (such as bladder smooth muscle cells, chondrocytes, osteoblasts, and vascular smooth muscle cells) respond differently to nano-structured PLGA surfaces (such as those with surface features less than 100 nm in at least one dimension) compared to nano-smooth surfaces. The purpose of the present in vitro research was to prepare PLGA films with various nanometer surface features and determine, for the first time, whether lung cancer epithelial cells respond differently to such topographies. Poly(dimethylsiloxane) (PDMS) molds prepared by placing PDMS onto various polystyrene monolayers and two solution evaporation methods were used to create nanometer surface features on PLGA. The intended spherical surface nano-topographies on PLGA with RMS values of 2.23, 5.03, 5.42 and 36.90 nm were formed, while PLGA surfaces with RMS values of 0.62 and 2.23 nm were obtained by different solution evaporation methods. Most importantly, lung cancer epithelial cells adhered less on the PLGA surfaces with an RMS value of 0.62, 2.23 and 5.42 nm after 4 hours of culture compared to any other PLGA surface created here. After three days, PLGA surfaces with an RMS value of 0.62 nm had much lower cell density than any other sample. In this manner, PLGA with specific nanometer surface features may inhibit lung cancer cell density which may provide for an important biomaterial for the treatment of lung cancer for a wide range of applications (from drug delivery to regenerative medicine).

Keywords

biomaterialnanostructurepolymer
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1209: Symposia P/YY – Business and Safety Issues in the Commercialization of Nanotechnology , 2009 , 1209-YY07-08
Copyright
Copyright © Materials Research Society 2010

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