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Cytotoxic and Anti-cancer Effects of Nickel Nanowires against Pancreatic Cancer Cells

Published online by Cambridge University Press:  30 March 2012

Md. Zakir Hossain ,
Wisam J. Khudhayer ,
Rozina Akter ,
Tansel Karabacak  and
Maurice G. Kleve
Md. Zakir Hossain*
Affiliation:
Applied Biosciences Emphasis, Department of Applied Science, University of Arkansas at Little Rock, Little Rock, AR-72204, United States
Wisam J. Khudhayer
Affiliation:
Department of Systems Engineering, University of Arkansas at Little Rock, Little Rock, AR-72204, United States
Rozina Akter
Affiliation:
Applied Biosciences Emphasis, Department of Applied Science, University of Arkansas at Little Rock, Little Rock, AR-72204, United States
Tansel Karabacak
Affiliation:
Department of Applied Science, University of Arkansas at Little Rock, Little Rock, AR-72204, United States
Maurice G. Kleve
Affiliation:
Department of Biology, University of Arkansas at Little Rock, Little Rock, AR-72204, United States
*
*Corresponding author e-mail: [email protected], [email protected]
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Abstract

Cytotoxicity study of magnetic nanomaterials is a key consideration for biomedical applications. Very little is known about the cytotoxic and anti-cancer effects of nickel nanowires (Ni NWs) on mammalian cells and their interaction with proliferating cancer cells. Current therapeutics do not address the full heterogeneity of pancreatic cancers due to the resistance to apoptosis and does not suffice for a successful treatment. Therefore, synthesis of novel anticancer drugs continues to be a potential topic for pancreatic cancer research. In this study, we have investigated the cellular toxicity and anti-cancer effects of Ni NWs in one of the most aggressive human pancreatic ductal cancer (Panc-1) cell lines with the objective of development of a potential treatment strategy. Ni NWs were fabricated in a custom-made setup utilizing the electrodeposition method. Elemental analysis, crystallographic structure, and morphological properties of the synthesized Ni NWs were investigated using Energy Dispersive X-ray Analysis (EDAX), X-Ray Diffraction (X-RD) and Scanning Electron Microscopy (SEM), respectively. Panc-1 cell cultures were maintained according to a slightly modified American Type Culture Collection (ATCC) protocol. Morphological apoptogenic characteristics assessment of the Ni NWs induced Panc-1 cell was accomplished using phase contrast microscopy (PCM). Two commercially available cytotoxicity procedures including 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) and trypan blue (TB) assays were utilized to determine the qualitative and quantitative cytotoxicity and anti-cancer effects of Ni NWs. As a negative control, Panc-1 cells without Ni NWs treatment were used in all experiments. Phase contrast microscopy (PCM) was used to confirm the Ni NWs internalization by Panc-1 cells. Both the MTT and TB assays, qualitatively and quantitatively confirmed the cytotoxic and anti-cancer effects of Ni NWs treated Panc-1 cells in vitro in both concentration and exposure-time dependent manners. We studied the cytotoxic and anti-cancer effects of Ni NWs on Panc-1 cells using novel integrated bionanotechnological approaches to understand the corresponding biological pathway with the objective of developing pancreatic cancer treatment. More specifically, we explored the molecular mechanisms associated with the pathway involved in Ni NWs induced toxicity against Panc-1 cells. Our results demonstrated that Ni NWs show strong candidacy for targeting cell selective applications in pancreatic cancer therapy. Key words: Nickel Nanowires, anti-cancer effects, pancreatic cancer.

Keywords

biomedicalNinanostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1416: Symposium JJ – Nanofunctional Materials, Nanostructures and Nanodevices for Cancer Applications , 2012 , mrsf11-1416-jj05-34
Copyright
Copyright © Materials Research Society 2012

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