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Covalent immobilization of DNA and hybridization on microchips by microsecond electric field pulses

Published online by Cambridge University Press:  15 March 2011

F. Fixe ,
H.M. Branz ,
D.M.F. Prazeres ,
V. Chu  and
J.P. Conde
F. Fixe
Affiliation:
INESC Microsistemas e Nanotecnologias, Lisbon, Portugal Center for Biological & Chemical Engineering, Instituto Superior Técnico, Lisbon, Portugal
H.M. Branz
Affiliation:
INESC Microsistemas e Nanotecnologias, Lisbon, Portugal National Renewable Energy Laboratory, Golden, CO, USA
D.M.F. Prazeres
Affiliation:
Center for Biological & Chemical Engineering, Instituto Superior Técnico, Lisbon, Portugal
V. Chu
Affiliation:
INESC Microsistemas e Nanotecnologias, Lisbon, Portugal
J.P. Conde
Affiliation:
INESC Microsistemas e Nanotecnologias, Lisbon, Portugal Department of Materials Engineering, Instituto Superior Técnico, Lisbon, Portugal
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Abstract

Single square voltage pulses were used to enhance by 7 and 9 orders of magnitude the rate of covalent immobilization and hybridization, respectively, of single stranded DNA probes on a chemically functionalized thin film surface (silicon dioxide) using 2 mm size electrodes. These electrodes were scaled down to 20 μm. Photolithography was used to define the electrode voltage line, ground line, and functionalized thin-film area on a plastic substrate (polyimide). At all electrode dimensions, electric field-assisted DNA immobilization and hybridization can be achieved in the microsecond time scale, far faster than the 2 hr or 16 hr needed for immobilization and hybridization, respectively, without the electric field. Pulse conditions optimized with the large-size electrodes (2 mm) were used in the microelectrodes.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 820: Symposia O/R – Nanoengineered Assemblies and Advanced Micro/Nanosystems , 2004 , O2.8
Copyright
Copyright © Materials Research Society 2004

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