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Construction of Biomimetic Environments with A Synthetic Peptide Analogue of Collagen.

Published online by Cambridge University Press:  15 February 2011

Rajendra S. Bhatnagar ,
Jing Jing Qian ,
Anna Wedrychowska  and
Nancy Smith
Rajendra S. Bhatnagar
Affiliation:
University of California, San Francisco, CA 94143-0424
Jing Jing Qian
Affiliation:
University of California, San Francisco, CA 94143-0424
Anna Wedrychowska
Affiliation:
University of California, San Francisco, CA 94143-0424
Nancy Smith
Affiliation:
University of California, San Francisco, CA 94143-0424
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Abstract

The flow of chemical and mechanical signals among cells, and between cells and their environment plays a crucial role in cell differentiation and morphogenesis. In tissues, type I collagen serves as the template for cell anchorage and migration, and it mediates the flux of regulatory signals via highly specific receptors. Cells respond to mechanical cues by secreting growth factors and remodeling their surrounding matrix in an exquisitely orchestrated spatial and temporal program of matrix turnover and organization. Cellular tractional forces contribute to the organization and orientation of the newly synthesized matrix, establishing the template for subsequent morphogenesis. The junction between cells and collagen plays a key role in cell differentiation, morphogenesis and tissue remodeling. An optimal biomimetic environment would emulate this pathway for the exchange of stimuli. To achieve this goal, we have constructed templates which place cells in apposition to P-15, a synthetic peptide ligand for collagen receptors. These environments prompted 3-D colony formation, induced increased osteogenic differentiation, and the deposition of highly oriented and organized matrix by human dermal and gingival fibroblasts and by osteoblast like HOS cells. These observations support our concept for biomimetic environments for tissue engineering.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 530: Symposium CC – Biomaterials Regulating Cell Function & Tissue Development , 1998 , 43
Copyright
Copyright © Materials Research Society 1998

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