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Conducting Polymer-based Aptamer Biosensor for in situ Monitoring of Cytokine

Published online by Cambridge University Press:  01 February 2011

Wei Liao ,
Bradly Randall ,
Nicolas Alba  and
Xinyan Tracy Cui
Wei Liao
Affiliation:
[email protected], University of Pittsburgh, Bioengineering, 5065 Biomedical Science Tower 3, 3501 Fifth ave, Pittsburgh, PA, 15260, United States
Bradly Randall
Affiliation:
[email protected], University of Pittsburgh, Bioengineering, Pittsburgh, PA, 15261, United States
Nicolas Alba
Affiliation:
[email protected], University of Pittsburgh, Bioengineering, Pittsburgh, PA, 15261, United States
Xinyan Tracy Cui
Affiliation:
[email protected], University of Pittsburgh, Bioengineering, Pittsburgh, PA, 15261, United States
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Abstract

Neural prostheses often suffer from undesired chronic inflammatory tissue response. Therefore; a sensitive and real-time biosensor for inflammatory cytokine detection would provide invaluable information toward solving this problem. Such a biosensor based on an aptamer probe doped in polypyrrole and subsequent electrochemical impedance spectroscopy (EIS) has been developed. PDGF, an important inflammatory cytokine, has been successfully measured in both offline EIS characterization and real-time impedance monitoring. For in situ detection of PDGF, the best sensitivity of 10 ng/ml has been achieved.

Keywords

biomedicalpolymerelectrochemical synthesis
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1065: Symposium QQ – Electroactive and Conductive Polymers and Carbon Nanotubes for Biomedical Applications , 2007 , 1065-QQ05-05
Copyright
Copyright © Materials Research Society 2008

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References

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