Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-23T06:22:30.252Z Has data issue: false hasContentIssue false
  • English
  • Français

Conductimetric Detection of Protein and Cancer Cells with Oxide Nanosensors

Published online by Cambridge University Press:  01 February 2011

Janagama Goud ,
P. Markondeya Raj ,
Jin Liu ,
Mahadevan Iyer ,
Z. L. Wang  and
Rao Tummala
Janagama Goud
Affiliation:
[email protected], Packaging Research Center, Electrical and Computer Engineering, 813 Ferst Drive NW, Atlanta, GA, 30329, United States, 404 894 2652
P. Markondeya Raj
Affiliation:
[email protected], Packaging Research Center, Georgia Institute of Technology, Atlanta, GA, 30332-0560, United States
Jin Liu
Affiliation:
[email protected], Packaging Research Center, Georgia Institute of Technology, Atlanta, GA, 30332-0560, United States
Mahadevan Iyer
Affiliation:
[email protected], Packaging Research Center, Georgia Institute of Technology, Atlanta, GA, 30332-0560, United States
Z. L. Wang
Affiliation:
[email protected], Packaging Research Center, Georgia Institute of Technology, Atlanta, GA, 30332-0560, United States
Rao Tummala
Affiliation:
[email protected], Packaging Research Center, Georgia Institute of Technology, Atlanta, GA, 30332-0560, United States
Get access

Abstract

Semiconducting oxides are widely known and commercially applied for their gas sensing properties. However, biochemical sensing has mostly depended on optical and electrochemical techniques that are more cumbersome. This work investigates the biosensing characteristics of ZnO nanobelts and ZnO thin films. Zinc oxide thin film sensors showed changes in conductivity after protein functionalization with rabbit IgG and hybridization with anti-rabbit IgG. Conductivity changes were also measured after coating the oxides with MCF-7 cancer cells and its antibodies. In another set of experiments, ZnO nanobelts showed systematic conductivity changes with rabbit IgG protein hybridization. The experimental results in this paper indicate that the conductimetric properties of nano and thin film oxides can be sensitized to protein and cancer cell hybridization reactions. This technique can also be applied to certain other pathogen proteins or toxic proteins from the environment leading to low-cost miniaturized wireless biosensors.

Keywords

nanostructuresemiconductingsensor
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1010: Symposium V – Functional Materials for Chemical and Biochemical Sensors , 2007 , 1010-V06-09
Copyright
Copyright © Materials Research Society 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Comini, E., Faglia, G. and Sberveglieri, G., Pan, Zhengwei and Wang, Zhong L., “Stable and highly sensitive gas sensors based on semiconducting oxide nanobelts”, Applied Physics Letters, Vol. 81, No. 10, Septeber 2002, pp. 1869–1871.Google Scholar
2. Hoffheins, B., “Solid State Resistive Gas Sensors,” in Hand Book of Chemical and Biological Sensors. Taylor, R.F. and Schultz, J.S., eds., Philadelphia: Institute of Physics, 1996.Google Scholar
3. Patolsky, F., Zheng, G. F., and Lieber, C. M., "Nanowire-Based Biosensors," Anal. Chem., vol. 78, pp. 4260–4269, Jul 2006.Google Scholar
4. Pan, Z. W., Dai, Z. R., and Wang, Z. L., "Nanobelts of Semiconducting Oxides," Science, vol. 291, p. 1947, 2001.Google Scholar
5. Souteyrand, E., Cloarec, J. P., Martin, J. R., Wilson, C., Lawrence, I., Mikkelsen, S., and Lawrence, M. F., "Direct Detection of the Hybridization of Synthetic Homo-Oligomer DNA Sequences by Field Effect," J. Phys. Chem. B, vol. 101, pp. 2980–2985, Apr 1997.Google Scholar
6. Zebda, A., Stambouli, V., Labeau, M., Matko, I., Chenevier, B., Renault, O., Guiducci, C., Chaudouet, P., Roussel, H., Nibkin, D., and Dupuis, E. “Metallic oxide CdIn2O4 films for the label free electrochemical detection of DNA hybridization”, Biosensors and Bioelectronics (Elsevier), (2006) vol. 22, pp. 178–18.Google Scholar
7. Calzolari, Arrigo, Felice, Rosa D, Molinari, Elisa and Garbesi, Anna, “Electron channels in biomolecular nanowires”, Journal of Physical Chemistry B, 2004, 108, 2509–2515.Google Scholar