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Complex Protein Patterns in Drying Droplets

Published online by Cambridge University Press:  01 February 2011

Ronald G. Gary Larson ,
Marc Alumnà López ,
Dong Woo Lim  and
Joerg Lahann
Ronald G. Gary Larson
Affiliation:
[email protected], University of Michigan, Chemical Engineering, Ann Arbor, Michigan, United States
Marc Alumnà López
Affiliation:
[email protected], University of Michigan, Chemical Engineering, Ann Arbor, Michigan, United States
Dong Woo Lim
Affiliation:
[email protected], University of Michigan, Chemical Engineering, Ann Arbor, Michigan, United States
Joerg Lahann
Affiliation:
[email protected], University of Michigan, Chemical Engineering, Ann Arbor, Michigan, United States
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Abstract

We demonstrate that deposition patterns formed during drying droplets of aqueous protein solutions are complex, characteristic, and highly reproducible. Substrate, buffer as well as protein type are important factors largely influencing the patterned structure. Specifically, multiple growth zones in what we refer to as “soccer ball pattern” are formed when a droplet of albumin solution in sodium bicarbonate buffer is dried. Each growth zone has periodically patterned, concentric ringed structures surrounding a core at the center. Different macroscopic patterns are also found for streptavidin, fibrinogen, IgG antibody as well as rhodamine B base and polystyrene beads when droplets of their aqueous solutions are dried on the substrates with different degrees of hydrophilicity/hydrophobicity. Furthermore, distinguishable deposition patterns are formed in drying droplets of aqueous protein solutions containing albumin and fibrinogen at different ratios, suggesting that even the relative abundance of multiple proteins influences the deposition patterns. Since the protein pattern is reproducible for a given protein and variable among different proteins, the protein patterns from drying droplets might be useful to potentially identify a given protein under specific conditions.

Keywords

biomaterialself-assemblysolution deposition
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1273: Symposium MM – Evaporative Self Assembly of Polymers, Nanoparticles, and DNA , 2010 , 1273-MM03-01
Copyright
Copyright © Materials Research Society 2010

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References

REFERENCES

1 Aizenberg, J. Advanced Materials 16, 1295 (2004).10.1002/adma.200400759Google Scholar
2 Aizenberg, J. Black, A.J. and Whitesides, G.M. Nature 398, 495 (1999).Google Scholar
3 Aizenberg, J. Lambert, G. Addadi, L. and Weiner, S. Advanced Materials 8, 222 (1996).Google Scholar
4 Aizenberg, J. Muller, D.A. Grazul, J.L. and Hamann, D.R. D. R. Science 299, 1205 (2003).Google Scholar
5 Aizenberg, J. Weaver, J.C. Thanawala, M.S. Sundar, V.C. Morse, D.E. Fratzl, P. P. Science 309, 275 (2005).Google Scholar
6 Gilbert, B. Frazer, B.H. Belz, A. Conrad, P.G. Nealson, K.H. Haskel, D. Lang, J.C. Srajer, G. and Stasio, G. De, Journal of Physical Chemistry A 107, 2839 (2003).10.1021/jp021493sGoogle Scholar
7 Gilbert, B. Huang, F. Lin, Z. Goodell, C. Zhang, H.Z. and Banfield, J.F. Nano Letters, 6, 605 (2006).10.1021/nl052201cGoogle Scholar
8 Gilbert, P. Abrecht, M. and Frazer, B.H. iMolecular Geomicrobiology 59, 157 (2005).Google Scholar
9 Han, Y.J. and Aizenberg, J. Journal of the American Chemical Society 125, 4032 (2003).10.1021/ja034094zGoogle Scholar
10 Pokroy, B. Kang, S.H. Mahadevan, L. and Aizenberg, J. Science, 323, 237 (2009).10.1126/science.1165607Google Scholar
11 Mann, S. Nature 332, 119 (1988).10.1038/332119a0Google Scholar
12 Hameren, R. van, Schon, P. van Buul, A.M., Hoogboom, J. Lazarenko, S.V. Gerritsen, J.W. Engelkamp, H. P.Christianen, C.M. Heus, H.A. Maan, J.C. Rasing, T. Speller, S. Rowan, A.E., Elemans, J. and R.Nolte, J.M. Science 314, 1433 (2006).10.1126/science.1133004Google Scholar
13 Deegan, R.D. Physical Review E 61, 475 (2000).Google Scholar
14 Deegan, R.D. Bkajin, O. Dupont, T.F. Huber, G. Nagel, S.R. and Witten, T.A. Nature 389, 827 (1997).10.1038/39827Google Scholar
15 Deegan, R.D. Bakajin, O. Dupont, T.F. Huber, G. Nagel, S.R. and Witten, T.A. Physical Review E 62, 756 (2000).Google Scholar
16 Hu, H. and Larson, R.G. Journal of Physical Chemistry B 106, 1334 (2002).10.1021/jp0118322Google Scholar
17 Hu, H. and Larson, R.G. Langmuir 21, 3963 (2005).10.1021/la047528sGoogle Scholar
18 Hu, H. and Larson, R.G. Langmuir 21, 3972 (2005).10.1021/la0475270Google Scholar
19 Hu, H. and Larson, R.G. Journal of Physical Chemistry B 110, 7090 (2006).10.1021/jp0609232Google Scholar
20 Maenosono, S. T. Okubo and Yamaguchi, Y. Journal of Nanoparticle Research 5, 5 (2003).10.1023/A:1024418931756Google Scholar
21 Tarasevich, Y.Y. and Pravoslavnova, D.M. Technical Physics 52, 159 (2007).Google Scholar
22 Tarasevich, Y.Y. and Pravoslavnova, D.M. European Physical Journal E 22, 311 (2007).Google Scholar
23 Kuncicky, D.M. Bose, K. Costa, K.D. and Velev, O.D. Chemistry of Materials 19, 141 (2007).10.1021/cm062217jGoogle Scholar
24 Kuncicky, D.M. and Velev, O.D. Langmuir 24, 1371 (2008).10.1021/la702129bGoogle Scholar
25 Cai, Y. and Newby, B.M.Z. Journal of the American Chemical Society 130, 6076 (2008).10.1021/ja801438uGoogle Scholar
26 Fan, F.Q. and Stebe, K.J. Langmuir 20, 3062 (2004).10.1021/la030380cGoogle Scholar
27 Hong, S.W. Jeong, W. Ko, H. Kessler, M.R. Tsukruk, V.V. and Lin, Z.Q. Advanced Functional Materials 18, 2114 (2008).10.1002/adfm.200800135Google Scholar
28 Maheshwari, S. Zhang, L. Zhu, Y. and Chang, H.-C. Phys. Rev. Lett. 100, 044503 (2008).10.1103/PhysRevLett.100.044503Google Scholar
29 Masuda, Y. Itoh, T. and Koumoto, K. Langmuir 21, 4478 (2005).10.1021/la050075mGoogle Scholar
30 Nguyen, V.X. and Stebe, K.J. Phys. Rev. Lett. 88, 164501 (2002).10.1103/PhysRevLett.88.164501Google Scholar
31 Shmuylovich, L. Shen, A.Q. and Stone, H.A. Langmuir 18, 3441 (2002).10.1021/la011484vGoogle Scholar
32 Takhistov, P. and Chang, H.C. Industrial & Engineering Chemistry Research 41, 6256 (2002).10.1021/ie010788+Google Scholar
33 Xu, J. Xia, J.F. and Lin, Z.Q. Angewandte Chemie-International Edition 46, 1860 (2007).Google Scholar
34 Zhang, L. Maheshwari, S. Chang, H.C. and Zhu, Y.X. Langmuir 24, 3911 (2008).Google Scholar
35 Chen, H.Y. Elkasabi, Y. and Lahann, J. Journal of the American Chemical Society 128, 374 (2006).10.1021/ja057082hGoogle Scholar
36 Chen, H.Y. Lai, J.H. Jiang, X.W. and Lahann, J. Advanced Materials 20, 3474 (2008).10.1002/adma.200800455Google Scholar
37 Elkasabi, Y. Chen, H.Y. and Lahann, J. Advanced Materials 18, 1521 (2006).Google Scholar
38 Elkasabi, Y. and Lahann, J. Macromolecular Rapid Communications 30, 57 (2009).10.1002/marc.200800578Google Scholar
39 Lahann, J. Hocker, H. and Langer, R. Angewandte Chemie-International Edition 40, 726 (2001).10.1002/1521-3773(20010216)40:4<726::AID-ANIE7260>3.0.CO;2-X3.0.CO;2-X>Google Scholar
40 Truskett, V. and Stebe, K.J. Langmuir 19, 8271 (2003).10.1021/la030049tGoogle Scholar
41 Chen, H.Y. McClelland, A.A. Chen, Z. and Lahann, J. Analytical Chemistry 80, 4119 (2008).10.1021/ac800341mGoogle Scholar
42 Elkasabi, Y. Yoshida, M. Nandivada, H. Chen, H.Y. and Lahann, J. Macromolecular Rapid Communications 29, 855 (2008).Google Scholar
43 Lahann, J. Polymer International 55, 1361 (2006).Google Scholar
44 Nandivada, H. Chen, H.Y. and Lahann, J. Macromolecular Rapid Communications 26, 1794 (2005).10.1002/marc.200500449Google Scholar
45 Hong, S.W. Byun, M. and Lin, Z.Q. Angewandte Chemie-International Edition 48, 512 (2009).10.1002/anie.200804633Google Scholar
46 Xu, J. Xia, J.F. Hong, S.W. Lin, Z.Q. Qiu, F. and Yang, Y.L. Physical Review Letters, 96, 066104 (2006).10.1103/PhysRevLett.96.066104Google Scholar
47 Wang, Y. Chan, C.M. Li, L. and Ng, K.M. Langmuir 22, 7384 (2006).10.1021/la060863rGoogle Scholar
48 Wang, Z.B. Hu, Z. Chen, Y.Z. Gong, Y.M. Huang, H.Y. and He, T.B. Macromolecules 40, 4381 (2007).10.1021/ma070334+Google Scholar
49 Henisch, H.K. Crystals in Gels and Liesegang Rings (Cambridge University Press: Cambridge, 1988)Google Scholar
50 Ristenpart, W.D. McCalla, P.M. Roy, R.V. and Stone, H.A. Physical Review Letters 97, 064501 (2006).10.1103/PhysRevLett.97.064501Google Scholar