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COMPARISON OF DEMINERALIZED AND DEPROTEINIZED BONE

Published online by Cambridge University Press:  04 February 2011

Ana B. Castro-Ceseña ,
Ekaterina Novitskaya ,
Po-Yu Chen ,
M. del Pilar Sánchez-Saavedra ,
Gustavo Hirata  and
Joanna McKittrick
Ana B. Castro-Ceseña
Affiliation:
Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Carretera Tijuana-Ensenada No. 3918, Zona Playitas, 22860, Ensenada, Baja California México
Ekaterina Novitskaya
Affiliation:
UC San Diego, 9500 Gilman Dr., La Jolla, CA 92093, U.S.A.
Po-Yu Chen
Affiliation:
UC San Diego, 9500 Gilman Dr., La Jolla, CA 92093, U.S.A.
M. del Pilar Sánchez-Saavedra
Affiliation:
Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Carretera Tijuana-Ensenada No. 3918, Zona Playitas, 22860, Ensenada, Baja California México
Gustavo Hirata
Affiliation:
Centro de Nanociencias y Nanotecnología, UNAM (CNyN-UNAM), Km 107 Carretera Tijuana-Ensenada, Apo. Postal, 356, CP. 22800, Ensenada, México.
Joanna McKittrick
Affiliation:
UC San Diego, 9500 Gilman Dr., La Jolla, CA 92093, U.S.A.
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Abstract

Cortical and cancellous bones were demineralized and deproteinized using 1 N HCl and 6% NaOCl, respectively. Experiments were performed at 37°C. The rate constants were calculated and the structural features of untreated and completely demineralized and deproteinized samples were studied by scanning electron microscopy, showing that intact, contiguous structures were obtained. For both cases, the rate constant was higher for cancellous bone than the cortical bone.

Keywords

bonekineticsscanning electron microscopy (SEM)
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1301: Symposium V/NN/OO/PP – Soft Matter, Biological Materials and Biomedical Materials—Synthesis, Characterization and Applications , 2011 , mrsf10-1301-nn01-07
Copyright
Copyright © Materials Research Society 2011

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References

REFERENCES

1. Chakkalakal, D. A., Strates, B. S., Garvin, K. L., Novak, J. R., Fritz, E. D., Moliner, T. J. and McGuire, M. H.. Tissue Eng. 7, 161 (2001).Google Scholar
2. Mauney, J. R., Jaquiéry, C., Volloch, V., Heberer, M., Martin, I. and Kaplan, D. L.. Biomater. 26, 3173 (2005).Google Scholar
3. Li, X., Jin, L., Balian, G., Laurencin, C. T. and Anderson, D. G.. Biomater. 27, 2426 (2006).Google Scholar
4. Wang, Z.-H., He, X.-J., Yang, Z.-Q. and Tu, J.-B.. Artif. Organs. 34, 161 (2010).Google Scholar
5. Dodds, R.A., York-Ely, A.M., Zhukauskas, R., Arola, T., Howell, J., Hartill, C., Cobb, R.R. and Fox, C.. J. Biomater. Appl. 25, 195 (2010).Google Scholar
6. Benke, D., Olah, A. and Möhler, H.. Biomaterials. 22, 1005 (2001).Google Scholar
7. Carter, D. H., Scully, A. J., Heaton, D. A., Young, M. P. J., and Aaron, J. E.. Bone. 31, 389 (2002).Google Scholar
8. Stavropoulos, A. and Karring, T.. J. Clin. Periodontol. 37, 200 (2010).Google Scholar
9. Lakes, R.. Nature 361, 511 (1993).Google Scholar
10. Rho, J-Y., Kuhn-Spearing, L. and Zioupos, P.. Med. Eng. Phys. 20, 92 (1998).Google Scholar
11. Weiner, S. and Wagner, H. D.. Annu. Rev. Mater. Sci. 28, 271 (1998).Google Scholar
12. Chen, P.-Y., Lin, A. Y. M., Lin, Y.-S., Seki, Y., Stokes, A.G., Peyras, J., Olevsky, E. A., Meyers, M.A. and McKittrick, J.. J. Mech. Behav. Biomed. Mater. I, 208 (2008).Google Scholar
13. Birkedal-Hansen, H.. J. Histochem. Cytochem. 22, 428 (1974).Google Scholar
14. Birkedal-Hansen, H.. J. Histochem. Cytochem. 22, 434 (1974).Google Scholar
15. Castro-Ceseña, A.B., Novitskaya, E.E., Chen, P.-Y., Hirata, G.A., and McKittrick, J.. Mat. Sci. Eng. C. (2010) doi: 10.1016/j.msec.2010.11.003.Google Scholar
16. Termine, J. D., Eanes, E. D., Greenfield, D. J., Nylen, M. U. and Harbor, R. A.. Calc. Tiss. Res. 12, 73 (1973).Google Scholar
17. Broz, J. J., Simske, S. J., Corley, W. D. and Greenberg, A. R.. J. Mater. Sci. - Mater. Med. 8, 395 (1997).Google Scholar
18. Fantner, G. E., Hassenkam, T., Kindt, J. H., Weaver, J. C., Birkedal, H., Pechenik, L., Cutroni, J. A., Cidade, G. A., Stuky, G. D., Morse, D. E. and Hansma, P. K.. Nat. Mater. 4, 612 (2005).Google Scholar
19. Thurner, P. J., Erickson, B., Turner, P., Jungmann, R., Lelujian, J., Proctor, A., Weaver, J. C., Schitter, G., Morse, D. E. and Hansma, P. K.. Adv. Mater. 21, 451 (2009).Google Scholar
20. Lowry, O. H., Rosebrough, N. J., Farr, A. l. and Randall, R. J.. J. Biol. Chem. 193, 265 (1951).Google Scholar
21. Bigi, A., Cojazzi, G., Panzavolta, S., Ripamonti, A., Roveri, N., Romanello, M., Noris Suarez, K. and Moro, L.. J. Inorg. Biochem. 68, 45 (1997).Google Scholar