Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-23T00:08:55.875Z Has data issue: false hasContentIssue false
  • English
  • Français

Chitosan-alginate Hybrid Scaffolds for in Vitro Bone Tissue Regeneration

Published online by Cambridge University Press:  01 February 2011

Zhensheng Li ,
Hassna R. Ramay ,
Kip D. Hauch  and
Miqin Zhang
Zhensheng Li
Affiliation:
Department of Materials Science & Engineering, University of Washington, Seattle, 302L Roberts Hall, WA 98195-2120, USA
Hassna R. Ramay
Affiliation:
Department of Materials Science & Engineering, University of Washington, Seattle, 302L Roberts Hall, WA 98195-2120, USA
Kip D. Hauch
Affiliation:
Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
Miqin Zhang
Affiliation:
Department of Materials Science & Engineering, University of Washington, Seattle, 302L Roberts Hall, WA 98195-2120, USA
Get access

Abstract

This paper reports the development of a biodegradable porous scaffold made from naturally derived chitosan and alginate polymers for bone tissue engineering. The scaffold has a 3-D interconnected porous structure and was fabricated through thermally induced phase separation. The mechanical test showed that the scaffold has compressive strength of 0.46 ± 0.02 MPa — about 4 times that of the pure chitosan scaffold. The cell-material interaction study indicated that osteoblast cells seeded on the chitosan-alginate scaffold attach and proliferate well. The mineral deposition occurred after 3 days of culture and formed large chunks after 7 days. The chitosan-alginate scaffold was also found to have desirable swelling property and was structurally stable in solutions of different pH. The chitosan-alginate scaffold can be prepared from solutions of neutral pH allowing growth factors to be incorporated homogeneously into the scaffold for sustained release. This research demonstrated a technique by which a polymer-based biodegradable scaffold can be made to have high porosity up to 92% and excellent mechanical and biological properties.

Keywords

chitosanalginatescaffoldbone tissuebiodegradable
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 873: Symposium K – Biological and Bio-Inspired Materials and Devices , 2005 , K8.2
Copyright
Copyright © Materials Research Society 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1 Jarcho, M., Calcium phosphate ceramics as hard tissue prosthetics. Clin Orthop, 1981(157): p. 259–78.Google Scholar
2 Hench, L.L. and Wilson, J., Surface-active biomaterials. Science, 1984. 226(4675): p. 630–6.Google Scholar
3 Yang, S., et al., The design of scaffolds for use in tissue engineering. Part I. Traditional factors. Tissue Eng, 2001. 7(6): p. 679–89.Google Scholar
4 Jolles, P., , R.A.A.M.E., Chitin and Chitinases. 1999, Birkhauser, Basel.Google Scholar
5 Madihally, S.V. and Matthew, H.W., Porous chitosan scaffolds for tissue engineering. Biomaterials, 1999. 20(12): p. 1133–42.Google Scholar
6 Thanoo, B.C., Sunny, M.C., and Jayakrishnan, A., Cross-linked chitosan microspheres: preparation and evaluation as a matrix for the controlled release of pharmaceuticals. J Pharm Pharmacol, 1992. 44(4): p. 283–6.Google Scholar
7 Park, Y.J., et al., Platelet derived growth factor releasing chitosan sponge for periodontal bone regeneration. Biomaterials, 2000. 21(2): p. 153–9.Google Scholar
8 Klokkevold, P.R., et al., steogenesis enhanced by chitosan (poly-N-acetyl glucosaminoglycan) in vitro. J Periodontol, 1996. 67(11): p. 1170–5.Google Scholar
9 Muzzarelli, C. and Muzzarelli, R.A., Natural and artificial chitosan-inorganic composites. J Inorg Biochem, 2002. 92(2): p. 89–94.Google Scholar
10 Shanmugasundaram, N., et al., Collagen-chitosan polymeric scaffolds for the in vitro culture of human epidermoid carcinoma cells. Biomaterials, 2001. 22(14): p. 1943–51.Google Scholar
11 Zhang, S., Emerging biological materials through molecular self-assembly. Biotechnol Adv, 2002. 20(5-6): p. 321–39.Google Scholar
12 Zhao, F., et al., Preparation and histological evaluation of biomimetic three-dimensional hydroxyapatite/chitosan-gelatin network composite scaffolds. Biomaterials, 2002. 23(15): p. 3227–34.Google Scholar
13 Zhang, Y. and Zhang, M., Three-dimensional macroporous calcium phosphate bioceramics with nested chitosan sponges for load-bearing bone implants. J Biomed Mater Res, 2002. 61(1): p. 1–8.Google Scholar
14 Zhang, Y. and Zhang, M., Calcium phosphate/chitosan composite scaffolds for controlled in vitro antibiotic drug release. J Biomed Mater Res, 2002. 62(3): p. 378–86.Google Scholar
15 Li, Z., et al., Chitosan-alginate hybrid scaffolds for bone tissue engineering. Biomaterials, 2005. 26(18): p. 3919–28.Google Scholar
16 Porter, B.D., et al., Mechanical properties of a biodegradable bone regeneration scaffold. J Biomech Eng, 2000. 122(3): p. 286–8.Google Scholar
17 Chu, T.M., et al., Mechanical and in vivo performance of hydroxyapatite implants with controlled architectures. Biomaterials, 2002. 23(5): p. 1283–93.Google Scholar
18 Thomson, R.C., et al., Fabrication of biodegradable polymer scaffolds to engineer trabecular bone. J Biomater Sci Polym Ed, 1995. 7(1): p. 23–38.Google Scholar
19 Vachoud, L., Zydowicz, N., and Domard, A., Physicochemical behaviour of chitin gels. Carbohydr Res, 2000. 326(4): p. 295–304.Google Scholar
20 Khalid, M.N., et al., Water state characterization, swelling behavior, thermal and mechanical properties of chitosan based networks. Eur J Pharm Sci, 2002. 15(5): p. 425–32.Google Scholar