Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-23T04:43:11.507Z Has data issue: false hasContentIssue false
  • English
  • Français

Characterizing the effect of substrate stiffness on neural stem cell differentiation

Published online by Cambridge University Press:  29 November 2012

Colleen T. Curley ,
Kristen Fanale  and
Sabrina S. Jedlicka
Colleen T. Curley
Affiliation:
Bioengineering Program, 111 Research Drive, Bethlehem, PA 18015, USA
Kristen Fanale
Affiliation:
Bioengineering Program, 111 Research Drive, Bethlehem, PA 18015, USA
Sabrina S. Jedlicka
Affiliation:
Bioengineering Program, 111 Research Drive, Bethlehem, PA 18015, USA Materials Science and Engineering Department, 5 E. Packer Avenue, Bethlehem, PA 18015, USA Center for Advanced Materials and Nanotechnology, 5 E. Packer Avenue, Bethlehem, PA 18015, USA
Get access

Abstract

Differentiated neurons (dorsal root ganglia and cortical neurons) have been shown to develop longer neurite extensions on softer materials than stiffer ones, but previous studies do not address the ability of neural stem cells to undergo differentiation as a result of material elasticity. In this study, we investigate neuronal differentiation of C17.2 neural stem cells due to growth on polyacrylamide gels of variable elastic moduli. Neurite growth, synapse formation, and mode of division (asymmetric vs. symmetric) were all assessed to characterize differentiation. C17.2 neural stem cells were seeded onto polyacrylamide gels coated with Type I collagen. The cells were then serum starved over a 14 day period, fixed, and analyzed for biochemical markers of differentiation. For division studies, time-lapse imaging of cells on various substrates was performed during serum withdrawal using the Nikon Biostation. Division events were analyzed using ImageJ to quantify sizes of resulting daughter. Data shows that C17.2 cell differentiation (as dictated by number and type of division events) is dependent upon substrate stiffness, with softer polyacrylamide surfaces (140 Pa) leading to increased populations of neurons and increased neurite length. Our data also indicates that the ability of neural stem cells to express synaptic proteins and develop synapses is dependent upon material elasticity.

Keywords

biomaterialbiomedicalelastic properties
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1498: Symposium L – Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications , 2013 , pp. 47 - 52
Copyright
Copyright © Materials Research Society 2012 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Discher, D.E., Mooney, D.J., Zandstra, P.W.., Science 324, 1673–77 (2009).CrossRefGoogle Scholar
Guilak, F., Cohen, D.M., Estes, B.T., Gimble, J.M., Liedtke, W., Chen, C.S.. Cell Stem Cell 5, 17–26 (2009).CrossRefGoogle Scholar
Hadjipanayi, E., Mudera, V., and Brown, R.A.., Cell Motil. Cytoskeleton 66, 121–128 (2009b).CrossRefGoogle Scholar
Lo, C.M., Wang, H.B., Dembo, M., and Wang, Y.L.., J. Biophys. 79, 144–152 (2000).CrossRefGoogle Scholar
Hadjipanayi, E., Mudera, V., and Brown, R.A.., J. Tissue Eng. Regen. Med. 3, 77–84 (2009a).CrossRefGoogle Scholar
Winer, J.P., Janmey, P.A., McCormick, M.E., and Funaki, M.., Tiss. Eng. Part A 15, 147–154 (2009).CrossRefGoogle Scholar
Engler, A.J., Griffin, M.A., Sen, S., Bonnetnann, C.G., Sweeney, H.L., and Discher, D.E.., J. Cell Biol. 166, 877–887(2004b).CrossRefGoogle Scholar
Engler, A.J., Sen, S., Sweeney, H.L., Discher, D.E.., Cell 126, 677–689 (2006).CrossRefGoogle ScholarPubMed
Yeung, T., Georges, P.C., Flanagan, L.A., Marg, B., Ortiz, M., Funaki, M., Zahir, N., Ming, W., Weaver, V., Janmey, P.A.., Cell motility and the Cytoskeleton 60, 24–34(2005).CrossRefGoogle Scholar
Flanagan, L.A., Ju, Y.E., Marg, B., Osterfield, M., Janmey, P.A.., Neuroreport 13, 2411–5 (2002).CrossRefGoogle Scholar
Pelham, R.J., and Wang, Y.L.., PNAS 94, 13661–5 (1997).CrossRefGoogle Scholar
Lu, B.W., Jan, L., and Jan, Y.N.., Annual Review of Neuroscience 23, 531–56 (2000).CrossRefGoogle Scholar
Snyder, E.Y., Deitcher, D.L., Walsh, C., Arnold-Aldea, S., Hartwieg, E.A., Cepko, C.L.., Cell 68, 33–51 (1992).CrossRefGoogle ScholarPubMed
Snyder, E.Y., Yoon, C.H., Flax, J.D., Macklis, J.D.., PNAS 94, 11663–8 (1997).CrossRefGoogle Scholar
Aplin, J. D. and Hughes, R. C.., J. Cell. Sci. 50, 89–103 (1981).Google Scholar
Johnson, K.R., Leight, J.L., Weaver, V.M.., Methods Cell Biol. 83, 547–83 (2007).CrossRefGoogle Scholar
Jiang, F.X., Yurke, B, Firestein, B.L., and Langrana, N.A.., Annals of Biomedical Engineering 36, 1565–1579 (2008).CrossRefGoogle Scholar
Meijering, E., Jacob, M., Sarria, J.-C. F., Steiner, P., Hirling, H., Unser, M.., Cytometry 58, 167–176(2004).CrossRefGoogle Scholar