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Characterization of a Micro Capillary Zone Electrophoresis System With Integrated Amorphous Silicon Based Optical Detectors

Published online by Cambridge University Press:  01 February 2011

Lars Schöler ,
Konstantin Seibel ,
Heiko Schäfer ,
René Johannes Püschl ,
Bernd Wenclawiak  and
Markus Böhm
Lars Schöler
Affiliation:
[email protected], University of Siegen, Institute for Microsystem Technologies, Hölderlinstr. 3, Siegen, N/A, Germany
Konstantin Seibel
Affiliation:
[email protected], University of Siegen, Institute for Microsystem Technologies, Hölderlinstr. 3, Siegen, N/A, Germany
Heiko Schäfer
Affiliation:
[email protected], University of Siegen, Institute for Microsystem Technologies, Hölderlinstr. 3, Siegen, N/A, Germany
René Johannes Püschl
Affiliation:
[email protected], University of Siegen, Analytical Chemistry, Adolf Reichwein Str. 2-4, Siegen, N/A, Germany
Bernd Wenclawiak
Affiliation:
[email protected], University of Siegen, Analytical Chemistry, Adolf Reichwein Str. 2-4, Siegen, N/A, Germany
Markus Böhm
Affiliation:
[email protected], University of Siegen, Institute for Microsystem Technologies, Hölderlinstr. 3, Siegen, N/A, Germany
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Abstract

Application specific Lab-on-Microchips (ALMs) making use of the combination of complex microfluidic networks with microelectronic circuits and micro optical components allow the realization of miniaturized application specific biological and chemical processing and analysis devices. Fluorescence sensing is one of the most widely used detection technologies, e.g. for DNA fluorescence labelling in Micro Capillary Electrophoresis (µCE) due to its superior sensitivity and specificity. Unfortunately, commercially available fluorescence sensing systems are physically very large, non portable, expensive and constrain the analysis in portable diagnostic and medical care. Integrated semiconductor optoelectronic devices can provide a portable, parallel and inexpensive solution for on chip fluorescence sensing.

Most µCE applications working in the spectral range of visible light. For the integration of optical detection components a photon energy range of 1.6 eV - 3.1 eV is of interest. The a-Si:H technology accomplished due to the low dark current and high absorption coefficient against to crystalline silicon the requirements in that spectral range. In this paper we combine a:Si-H photo sensors with a fluidic micro system to detect the fluorescence of a rhodamine analyte mixture. The analyte mixture was excited by light with a wavelength in the range of λEx = 450 - 490 nm. The a-Si:H detector reveals a low dark current density on the order of 10-10 A/cm2 and a sufficient dynamic range of ∼100 dB under illumination of ∼1000 lx as a function of bias voltage. The measurement shows that the movement of the rhodamine plug in the microchannel causes a significant rise in the pin-diode photo current, which correlates to the evaluated signal of a microscope image detector. The photo current difference for excitation and additional fluorescence amounts to 2.4 µA.

Keywords

electromigrationmicrostructurefluidics
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1004: Symposium P – Materials and Strategies for Lab-on-a-Chip-Biological Analysis, Microfactories, and Fluidic Assembly of Nanostructures , 2007 , 1004-P03-20
Copyright
Copyright © Materials Research Society 2007

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