Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-27T01:41:37.705Z Has data issue: false hasContentIssue false
  • English
  • Français

Biomimetic Poly(L-Lactic Acid) Scaffolds with Interconnected Macropores, Collagen-Like Nano-Scale Fibers, and Bone-Like Apatite

Published online by Cambridge University Press:  11 February 2011

Victor J. Chen  and
Peter X. Ma
Victor J. Chen
Affiliation:
Department of Biomedical Engineering, Ann Arbor, MI 48109–1078, U.S.A.
Peter X. Ma
Affiliation:
Department of Biomedical Engineering, Ann Arbor, MI 48109–1078, U.S.A. Biologic and Materials Sciences, Ann Arbor, MI 48109–1078, U.S.A. Macromolecular Science and Engineering Center, Ann Arbor, MI 48109–1078, U.S.A.
Get access

Abstract

Highly porous nano-fibrous poly(L-lactic acid) (PLLA) scaffolds were prepared and incubated in a simulated body fluid which resulted in the in situ formation of partially carbonated bone-like apatite. The macroporous interconnected architecture was created with a three-dimensional mold of thermally-bonded paraffin microspheres. PLLA solution was cast over the paraffin mold and was thermally induced to phase separate to form nano-scale fibers that mimic the fibrous structures of type I collagen. To potentially improve the osteoconductivity of these scaffolds, they were incubated in a buffered simulated body fluid at 37°C for certain periods of time to allow for apatite formation. It was seen that over time, the apatite particles increased in size. In addition, the growth of apatite particles on the nano-fibrous scaffolds was compared with the growth of particles on similar porous PLLA scaffolds with a solid-walled (not nano-fibrous) morphology. It was seen that the initial number of particles per unit area and the overall increase in mass of the scaffolds were significantly higher in the nano-fibrous scaffolds than in the solid-walled scaffolds. These novel scaffolds have well-defined architectures at three different size scales: (i) interconnected spherical pores ∼250–400 m in diameter; (ii) fibrous collagen-like matrix with fibers 50–500 nm in diameter; and (iii) carbonated bone-like apatite particles at the nanometer to micrometer scales; the scaffolds may serve as superior support for bone tissue regeneration.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 735: Symposium C – Bioinspired Nanoscale Hybrid Systems , 2002 , C6.5
Copyright
Copyright © Materials Research Society 2003

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Langer, R., Vacanti, J., Science 260, 920 (1993).Google Scholar
2. Yaszemski, M., Payne, R., Langer, R., Mikos, A., Biomaterials 17, 175 (1996).Google Scholar
3. Crane, G., Ishaug, S., Mikos, A., Nat. Med. 1, 1322 (1995).Google Scholar
4. Vacanti, C., Vacanti, J. Otolaryngol. Clin. N. Am. 27, 263 (1994).Google Scholar
5. Yang, S., Leong, K., Du, Z., Chua, C., Tissue Eng. 8, 1 (2002).Google Scholar
6. Ma, P., Choi, J., Tissue Eng. 7, 23 (2001).Google Scholar
7. Zhang, R., Ma, P., J. Biomed. Mater. Res. 45, 285 (1999).Google Scholar
8. Ma, P., Zhang, R., J. Biomed. Mater. Res. 46, 60 (1999).Google Scholar