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Bio-Hybrid Materials for Immunoassay-Based Sensing

Published online by Cambridge University Press:  01 February 2011

Jonathan Fang ,
Esther H. Lan ,
Jing C. Zhou  and
Bruce Dunn
Jonathan Fang
Affiliation:
[email protected] of California, Los AngelesDepartment of Biomedical Engineering IDPLos Angeles CA 90095United States
Esther H. Lan
Affiliation:
[email protected], University of California, Los Angeles, Department of Materials Science and Engineering, Los Angeles, CA, 90095, United States
Jing C. Zhou
Affiliation:
[email protected], University of California, Los Angeles, Department of Biomedical Engineering IDP, Los Angeles, CA, 90095, United States
Bruce Dunn
Affiliation:
[email protected], University of California, Los Angeles, Department of Materials Science and Engineering, Los Angeles, CA, 90095, United States
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Abstract

The process of encapsulating antibodies in sol-gel was used for sensing various hormones, specifically cortisol, insulin, and C-peptide. A sol-gel optical biosensor for cortisol has been developed for monitoring of crew health on-orbit during space missions. Our studies involving silica sol-gel materials with competitive immunoassays demonstrated linear calibration for cortisol in the range of 2-60 μg/dL, which covers the physiological range of cortisol blood concentration for an adult (2-28 μg/dL). The method of standard additions was used to analyze human serum samples sent to us from a NASA laboratory. Our sol-gel immunosensor values were typically within 20% of the values obtained by NASA-JSC using traditional immuno-binding techniques, with some values having less than a 5% error. Initial results are presented for sensing the hormones insulin and C-peptide.

Keywords

sol-gelbiomedicalsensor
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 915: Symposium R – Nanostructured Materials and Hybrid Composites for Gas Sensors and Biomedical Applications , 2006 , 0915-R01-01
Copyright
Copyright © Materials Research Society 2006

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References

1 Dave, B.C., Dunn, B., Valentine, J.S., Zink, J.I., Anal. Chem. 66, A1120 (1994)Google Scholar
2 Lan, E.H, Dunn, B., Zink, J.I., Chem. Mater. 12, 1874 (2000)Google Scholar
3 Li, C.-I., Lin, Y.-H., Shih, C.-L., Tsaur, J.-P., Chau, L.-K., Biosensors and Bioelectronics 17, 323 (2002)Google Scholar
4 Doong, R.-A., Tsai, H.-C., Analytica Chimica Acta 434, 239 (2001)Google Scholar
5 Okada, R., Yamamoto, K., Koda, A., et al., General and Comparative Endocrinology 135, 42 (2004)Google Scholar
6 Morinelli, T.A., Meier, G.P., Webb, J.G., et al., International Immunopharmacology 2, 1995 (2002)Google Scholar
7 Niu, P.D., Molecular and Cellular Endocrinology 162, 131 (2000)Google Scholar
8 Lewis, J.G., Elder, P.A., Journal of Steroid Biochemistry 2, 673 (1985)Google Scholar
9 Lewis, J.G., Manley, L., Townsend, J.C., Elder, P.A., Clinica Chimica Acta 159, 205 (1986)Google Scholar
10 Dunn, B., Miller, J.M., Dave, B.C., Valentine, J.S., Zink, J.I., Acta mater 46, 737 (1998)Google Scholar
11 Livage, J., Beteille, F., Roux, C., Chatry, M., Davidson, P., Acta mater 46, 743 (1998)Google Scholar
12 Livage, J., Sanchez, C., Henry, M., Doeuff, S., Solid State Ionics 32–33, 633 (1989)Google Scholar
13 Tortora, G., Grabowski, S.R., Principles of Anatomy & Physiology, 10th ed. (John Wiley & Sons, Inc., New York, 2003) p. 611–612 Google Scholar
14 Borchers, A.T., Keen, C.L., Gershwin, M.E., Nutrition 18, 889 (2002)Google Scholar
15 Zhou, J.C., Chuang, M.H., Lan, E.H., Dunn, B., Gillman, P.L., Smith, S.M., J. Mater. Chem. 14, 2311 (2004)Google Scholar
16 Hori, Y., Gu, X., Xie, X., Kim, S.K., PLoS Medicine 2, 347 (2005)Google Scholar
17 Lumelsky, N., Blondel, O., Laeng, P., Velasco, I., Ravin, R., McKay, R., Science 292, 1389 (2001)Google Scholar
18 Hund, E., Massart, D.L., Smeyers-Verbeke, J., Journal of Pharmaceutical and Biomedical Analysis 21, 23 (1999)Google Scholar
19 Li, H., Analytical Letters 24, 473 (1991)Google Scholar
20 Yperman, J., Backer, A.D., Vos, A., Franco, D., Mullens, J., Poucke, L.C. Van, Analytica Chimica Acta 511 (1993)Google Scholar