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Biodegradable Polymeric Nanoparticles for Tumor-Selective Tamoxifen Delivery: In Vitro and in Vivo Studies

Published online by Cambridge University Press:  01 February 2011

Dinesh B. Shenoy ,
Jugminder S. Chawla  and
Mansoor M. Amiji
Dinesh B. Shenoy
Affiliation:
Department of Pharmaceutical Sciences, School of Pharmacy, Northeastern University, Boston, MA 02115
Jugminder S. Chawla
Affiliation:
Department of Pharmaceutical Sciences, School of Pharmacy, Northeastern University, Boston, MA 02115
Mansoor M. Amiji*
Affiliation:
Department of Pharmaceutical Sciences, School of Pharmacy, Northeastern University, Boston, MA 02115
*
* Corresponding author: Tel. 617-373-3137, Fax. 617-373-8886, Email: [email protected]
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1. ABSTRACT: This study was performed to evaluate the in-vitro and in-vivo tumor-cellular uptake and biodistribution pattern of tamoxifen when administered intravenously as a simple solution and upon encapsulation into biodegradable, surface-modified poly(ε-caprolactone) (PCL) nanoparticles. PCL (MW ∼ 15, 000) nanoparticles were prepared by the solvent displacement method and characterized for particle size/charge and surface morphology (by scanning electron microscopy). We investigated the nanoparticle-surface modification potential of the hydrophilic stabilizer (Pluronic® F-68 and F-108) employed during the preparation by electron spectroscopy for chemical analysis (ESCA). Quantitative in-vitro cellular uptake of tritiated (3H) tamoxifen in solution form and as nanoparticulate formulation was assessed in MCF-7 breast cancer cells. In-vivo biodistribution studies for the same formulations were carried out in Nu/Nu mice bearing MDA-MB-231 human breast carcinoma xenograft. Spherical nanoparticles having positive zeta potential (∼25 mV) were obtained in the size range of 200-300 nm. Pluronics (both F-68 and F-108), the triblock copolymers of poly(ethylene oxide) (PEO) and poly(propylene oxide) induced surface hydrophilization of the nanoparticles via adsorption as evident by ESCA. Nanoparticulate formulations of tamoxifen achieved higher intracellular concentrations when exposed at therapeutic concentrations to tumor cells in-vitro compared to solutions. The in-vivo biodistribution studies carried out in nude mice bearing experimental breast tumor suggested increased tumor concentrations for the drug administered as nanoparticulate formulations besides longer retention times within tumor mass. This type of delivery system is expected to provide better therapeutic benefit by dual means: preferential concentration within the tumor mass via enhanced permeation and retention pathway, and; subsequent controlled release, thus maintaining the local drug concentration for longer periods of time to achieve maximal cell-kill.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 845: Symposium AA – Nanoscale Materials Science in Biology and Medicine , 2004 , AA5.49
Copyright
Copyright © Materials Research Society 2005

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References

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