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Bioconjugates of Luminescent CdSe-ZnS Quantum Dots with Engineered Recombinant Proteins: Novel Self-Assembled Tools for Biosensing

Published online by Cambridge University Press:  17 March 2011

Ellen R. Goldman ,
Hedi Mattoussi ,
Phan T. Tran ,
George P. Anderson  and
J. Matthew Mauro
Ellen R. Goldman
Affiliation:
Center for Bioresource Development, George Mason University Center for Bio/Molecular Science and Engineering, Washington, DC 20375, U.S.A.
Hedi Mattoussi
Affiliation:
Division of Optical Sciences Washington, DC 20375, U.S.A. Also Sachs Freeman Assoc. Inc., Largo, MD 20774
Phan T. Tran
Affiliation:
Center for Bio/Molecular Science and Engineering, Washington, DC 20375, U.S.A.
George P. Anderson
Affiliation:
Center for Bio/Molecular Science and Engineering, Washington, DC 20375, U.S.A.
J. Matthew Mauro
Affiliation:
Center for Bio/Molecular Science and Engineering, Washington, DC 20375, U.S.A.
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Abstract

Colloidal semiconductor quantum dots (QDs) are luminescent nanoparticles with size- dependent emission spectra spanning a wide range of wavelengths in the visible and near IR. This property, as well as their higher resistance to photo-degradation compared to organic dye labels, makes QDs potentially suitable for certain biomolecule tagging and multiplexing applications. We describe an electrostatic self-assembly approach for conjugating highly luminescent colloidal CdSe-ZnS core-shell QDs with engineered two-domain recombinant proteins to form conjugates for sensing and imaging applications. The design, preparation, and characterization of high quantum yield IgG antibody-binding protein G bioconjugates using luminescence, electrophoretic gel shift, and affinity assays is reported.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 642: Symposium J – Semiconductor Quantum Dots II , 2000 , J2.8.1
Copyright
Copyright © Materials Research Society 2001

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