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Artificial Protein Hydrogel Materials

Published online by Cambridge University Press:  15 February 2011

W. A. Petka ,
J. L. Harden ,
J. K. Sakata  and
D. A. Tirrell
W. A. Petka
Affiliation:
Department of Polymer Science and EngineeringUniversity of Massachusetts, Amherst, MA 01003
J. L. Harden
Affiliation:
Department of Chemical EngineeringJohns Hopkins University, Baltimore, MD 21218
J. K. Sakata
Affiliation:
Department of Polymer Science and EngineeringUniversity of Massachusetts, Amherst, MA 01003 Division of Chemistry and Chemical EngineeringCalifornia Institute of Technology, Pasadena, CA 91125
D. A. Tirrell
Affiliation:
Division of Chemistry and Chemical EngineeringCalifornia Institute of Technology, Pasadena, CA 91125
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Abstract

Recombinant DNA methods were used to create a new class of artificial proteins that undergo reversible gelation in response to changes in pH or temperature. These proteins consist of terminal a-helical “leucine zipper” domains flanking a central, water-soluble polvelectrolyte segment. The formation of coiled-coil aggregates of the terminal domains in near-neutral pH solution triggers formation of a polymer hydrogel, with the central polyelectrolyte segment retaining solvent and preventing precipitation of the chains. Dissociation of the coiled-coil aggregates through elevation of pH or temperature causes dissolution of the gel and a return to the viscous behavior characteristic of a polymer solution. The pH and temperature range of the hydrogel state and its viscoelastic properties may be systematically varied through precise changes of the length, composition and charge density of the terminal and central blocks. Such control is of value in designing hydrogels with predetermined physical properties and makes these biosynthetic triblock copolymer systems attractive candidates for use in molecular and cellular encapsulation and in controlled reagent delivery.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 550: Symposium GG/HH/II – Biomedical Materials-Drug Delivery, Implants and Tissue Engr , 1998 , 23
Copyright
Copyright © Materials Research Society 1999

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References

[1] Protein-Based Materials, edited by McGrath, K. and Kaplan, D. (Birkhauser, Boston, MA 1997).Google Scholar
[2] Guenet, J.-M., Thermoreversible Gelation of Polymers and Biopolymers (Academic Press, London, 1992).Google Scholar
[3] Hydrogels and Biodegradable Polymers for Bioapplications, edited by Ottenbrite, R. M., Huang, S. J., and Park, K. (American Chemical Society, Washington, D.C., 1996).Google Scholar
[4] Winnik, M. A., Yekta, A., Curr. Opin. Colloid Interface Sci. 2, 424 (1997).Google Scholar
[5] Landshultz, W. H., Johnson, P. F., McKnight, S. L., Science 240, 1759 (1988).Google Scholar
[6] Harbury, P. B., Zhang, T., Kim, P. S., Alber, T., Science 262, 1401 (1993).Google Scholar
[7] Lumb, K. J. and Kim, P. S., Science 268, 436 (1995);Google Scholar
O'Shea, E. K., Rutkowski, R., Stafford, W. F. III, Kim, P. S., Science 245, 646 (1989).Google Scholar
[8] Lau, S. Y. M., Taneja, A. K., Hodges, R. S., J. Biol. Chem. 259, 13253 (1984).Google Scholar
[9] O'Shea, E. K., Rutkowski, R., Kim, P. S., Cell 68, 699 (1992).Google Scholar
[10] Lupas, A., Dyke, M. V., Stock, J., Science 252, 1162 (1991).Google Scholar
[11] McGrath, K. P., Fournier, M. J., Mason, T. L., Tirrell, D. A., J. Am. Chem. Soc. 114, 727 (1992).Google Scholar
[12] Petka, W. A., PhD thesis, University of Massachusetts, Amherst, 1997.Google Scholar
[13] Petka, W. A., Harden, J. L., McGrath, K. P., Wirtz, D., and Tirrell, D. A., Science 281, 389 (1998).Google Scholar
[14] Weitz, D. A., Pine, D. J., in Dynamic Light Scattering; The Method and Some Applications, edited by Brown, W. (Clarendon, Oxford, 1993), p. 719.Google Scholar
[15] Mason, T. C., Weitz, D. A., Phys. Rev. Lett. 74, 1252 (1995);Google Scholar
Mason, T. G., Gang, H., Weitz, D. A., J. Mol. Struct. 81, 383 (1996).Google Scholar