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Arrayed Nanohydrogels for Protein Array Technology

Published online by Cambridge University Press:  26 February 2011

Ishtiaq Saaem ,
Vasilis Papasotiropoulos ,
Tongsheng Wang ,
Patricia Soteropoulos  and
Matthew Libera
Ishtiaq Saaem
Affiliation:
[email protected], Stevens Institute of Technology, United States
Vasilis Papasotiropoulos
Affiliation:
[email protected], Center for Applied Genomics
Tongsheng Wang
Affiliation:
[email protected], Center for Applied Genomics
Patricia Soteropoulos
Affiliation:
[email protected], Center for Applied Genomics
Matthew Libera
Affiliation:
[email protected], Stevens Institute of Technology
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Abstract

This paper describes studies of surface-patterned nanohydrogels and their use as possible substrates for high-density and high-sensitivity protein arrays. Nanohydrogels, approximately 200 nm in diameter, were created by locally crosslinking dry amine-terminated poly(ethylene glycol) [PEG] (5000 Da) thin films using a focused electron beam. These gels then had a dry height of 50 nm and a swell ratio of about five. They were patterned into arrays with approximately 1 μm inter-gel spacing. These arrayed gels were functionalized with Zinc Finger 9 (ZNF9), a nucleic-acid binding protein. As part of ongoing research, we are interested in how the performance of the functionalized gels compares to that of current commercial substrates. We show, using an assay that binds α-GST antibody to mediate attachment of GST-tagged ZNF9, that nanohydrogels have a consistently higher combination of fluorescent signal and signal-to-noise (SNR) ratio than the comparable commercial substrates. We further show that the SNR characteristic of our assay degrades slower on the nanohydrogel array than on a comparable microarray printed on an epoxide substrate, and we speculate that the difference is in part attributable to the fact that the nanohydrogel array presents a softer and more hydrophilic surface.

Keywords

nanoscaleelectron irradiationpolymer
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 897: Symposium J – Biomemetic Polymers and Gels , 2005 , 0897-J06-07
Copyright
Copyright © Materials Research Society 2006

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