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Zinc Oxide Nanorod Films for Electrochemical Urea Biosensor

Published online by Cambridge University Press:  03 August 2011

Netzahualcóyotl Palomera ,
Marcia Balaguera ,
Sunil K. Arya ,
Samuel Hernández ,
Maharaj S. Tomar ,
Jaime E. Ramírez-Vick  and
Surinder P. Singh
Netzahualcóyotl Palomera
Affiliation:
Department of Mechanical Engineering, University of Puerto Rico, Call Box 9000, Mayagüez, PR 00681-9000.
Marcia Balaguera
Affiliation:
Department of Chemistry, University of Puerto Rico, Call Box 9000, Mayagüez, PR 00681-9000.
Sunil K. Arya
Affiliation:
Bio-MEMS and Microsystem Lab, Department of Electrical Engineering, University of South Florida, Tampa, FL 33620, United States.
Samuel Hernández
Affiliation:
Department of Chemistry, University of Puerto Rico, Call Box 9000, Mayagüez, PR 00681-9000.
Maharaj S. Tomar
Affiliation:
Department of Physics, University of Puerto Rico, Call Box 9000, Mayagüez, PR 00681-9000.
Jaime E. Ramírez-Vick
Affiliation:
Department of Engineering Science & Materials, University of Puerto Rico, Call Box 9000, Mayagüez, PR 00681-9000.
Surinder P. Singh
Affiliation:
Department of Engineering Science & Materials, University of Puerto Rico, Call Box 9000, Mayagüez, PR 00681-9000.
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Abstract

Metal oxide nanostructures have shown significant promise for biosensors, gas sensors, photocatalyst and other biomedical applications. Among these, zinc oxide (ZnO) nanostructures, exhibiting interesting properties such as high catalytic activity, biocompatibility, high isoelectric point, large surface to volume ratio, make them a good candidate for biosensing applications. Here we report the synthesis of ZnO nanorods (ZnONR) on ITO films in aqueous phase and its application in Urea biosensor fabrication. ZnONR have been synthesized by a two-step method, first seed growth of ZnO by sputtering on ITO films followed by decomposition of zinc nitrate hexahydrate / hexamethylenetetramine (HMT) in aqueous phase. Exploiting the high isoelectric point of ZnO, a Urease/ZnONR/ITO bioelectrode has been fabricated by physical binding of Urease (Urs) onto ZnONRs. X-ray diffraction (XRD), field emission-scanning electron microscopy (FE-SEM), and cyclic voltammetry (CV) have been used to characterize ZnONR and the Urs/ZnONR/ITO bioelectrode. The FE-SEM and XRD measurements confirm the formation of ZnONR. The electrochemical data from the Urs/ZnONR/ITO biolectrode reveal linearity between 1-11 mM with sensitivity of 0.9 μA/mM and a relatively low Michaelis-Menten constant (Km) of 5.01 mM for urea sensing. The results indicate the potential of ZnONR films for fabrication of commercial biosensors.

Keywords

biomaterialII-VInanostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1355: Symposium JJ – Biological Hybrid Materials for Life Sciences , 2011 , mrss11-1355-jj09-16
Copyright
Copyright © Materials Research Society 2011

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References

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