Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-09T15:40:07.208Z Has data issue: false hasContentIssue false

Young’s Modulus of Cortical and P19 Derived Neurons Measured by Atomic Force Microscopy

Published online by Cambridge University Press:  16 March 2012

Elise Spedden
Affiliation:
Department of Physics and Astronomy, Tufts University, 4 Colby St, Medford Ma 02155
James D. White
Affiliation:
Department of Biomedical Engineering, Tufts University, 4 Colby St, Medford Ma 02155
David Kaplan
Affiliation:
Department of Biomedical Engineering, Tufts University, 4 Colby St, Medford Ma 02155 Department of Chemical Engineering, Tufts University, 4 Colby St, Medford Ma 02155
Cristian Staii
Affiliation:
Department of Physics and Astronomy, Tufts University, 4 Colby St, Medford Ma 02155
Get access

Abstract

In this paper we use the Atomic Force Microscope to measure the Young’s modulus for two types of neuronal cell bodies: cortical neurons obtained from rat embryos and neurons derived from P19 mouse embryonic carcinoma stem cells. The neurons are plated on different substrates coated with two types of protein growth factors, poly-D-lysine and laminin. We report on the Young’s modulus of each type of neuron as well as the variation of modulus between cells plated on different protein substrates. We compare these results to various individual cell and bulk tissue measurements reported in literature. We additionally report on an observed change in the Young’s modulus of cortical neurons when subjected to a short-term reduction in ambient temperature.

Type
Research Article
Copyright
Copyright © Materials Research Society 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Staii, C., Viesselmann, C., Ballweg, J., Williams, J. C., Dent, E. W., Coppersmith, S. N., and Eriksson, M. A., “Distance Dependence of Neuronal Growth on Nanopatterned Gold Surfaces,” Langmuir 2011 27(1), 233239.Google Scholar
2. Messa, M., Canale, C., Marconi, E., Cingolani, R., Salerno, M., and Benfenati, F., “Growth cone 3-D morphology is modified by distinct micropatterned adhesion substrates,” IEEE Trans Nanobioscience. 2009 Jun; 8(2):161–8.Google Scholar
3. Costa, K.D., “Single-cell elastography: probing for disease using the atomic force microscope,” Dis Markers, 19 (2003), pp. 139154.Google Scholar
4. Elkin, B.S., Azeloglu, E.U., Costa, K.D., and Morrison, B. 3rd, “Mechanical heterogeneity of the rat hippocampus measured by atomic force microscope indentation,” J Neurotrauma. 2007May; 24(5):812–22.Google Scholar
5. Lu, Y.B., Franze, K, Seifert, G, Steinhauser, C, Kirchoff, F, Wolburg, H, Guck, J, Janmey, P, Wei, E, Kas, J, and Reichenbach, A, “Viscoelastic properties of individual glial cells and neurons in the CNS,” Proc Natl Acad Sci USA, (2006) 103:1775917764.Google Scholar
6. Carl, P., Schillers, H., “Elasticity measurement of living cells with an atomic force microscope: data acquisition and processing,” Pflugers Arch. 2008 Nov; 457(2):551–9.Google Scholar
7. Kuznetsova, TG, Starodubtseva, MN, Yegorenkov, NI, Chizhik, SA, and Zhdanov, RI, “Atomic force microscopy probing of cell elasticity,” Micron. 2007; 38(8):824–33.Google Scholar
8. Leventhal, A., Georges, P. and Janmey, P.Soft biological materials and their impact on cell function”, Soft Matter., vol. 3, p.299, 2007.Google Scholar
9. Mustata, M, Ritchie, K, and McNally, HA, “Neuronal elasticity as measured by atomic force microscopy,” J Neurosci Methods. 2010 Jan 30;186(1):3541.Google Scholar