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X-Ray Absorption Spectroscopy of Nitrogenase Mo-Fe Protein

Published online by Cambridge University Press:  15 February 2011

J. Chen ,
J. Christiansen ,
S. George ,
R. Tittsworth ,
B. Hales ,
N. Campobasso ,
J. Bolin  and
S. P. Cramer
J. Chen
Affiliation:
Department of Applied Science, University of California, Davis, CA 95616
J. Christiansen
Affiliation:
Department of Applied Science, University of California, Davis, CA 95616
S. George
Affiliation:
Department of Applied Science, University of California, Davis, CA 95616
R. Tittsworth
Affiliation:
Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803
B. Hales
Affiliation:
Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803
N. Campobasso
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907.
J. Bolin
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907.
S. P. Cramer
Affiliation:
Department of Applied Science, University of California, Davis, CA 95616
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Abstract

We have studied both the atomic and electronic structures of nitrogenase Mo-Fe proteins using EXAFS and L-edge spectroscopy. The Mo and Fe K-edge EXAFS data show not only 2.37, 2.70, 2.29, and 2.63 Å first shell Mo-S, Mo-Fe, Fe-S, and Fe-Fe distances, but also 3.8, 4.3 and 5.1 Å second shell Fe-Fe, Fe-S, and Mo-Fe interactions. These observed distances are consistent with recent crystallographic models for the M-center. The average Mcenter distances have been defined with unprecedented accuracy. The nitrogenase Fe L-edge spectra have been obtained for the first time, and the result suggests that the Fe exists in both Fe2+ and Fe3+ states.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 307: Symposium L – Applications of Synchrotron Radiation Techniques to Materials Science , 1993 , 63
Copyright
Copyright © Materials Research Society 1993

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References

REFERENCES

1. Orme-Johnson, W.H., Ann. Rev. Biophys. Biophys. Chem. 14, 419 (1985); F.B. Simpson and R.H. Burns, Science, 224, 1095 (1984).Google Scholar
2. Shah, V.K. and Brill, W.J., Proc. Natl. Acad. Sci. U.S.A. 74, 3249 (1977).Google Scholar
3. Kim, J. and Rees, D.C., Science, 257, 1653 (1992);Google Scholar
4. Kim, J. and Rees, D.C., Science, 257, 1677 (1992); Nature, 360, 553 (1992); J.T. Bolin, A.E. Ronco, T.V. Morgan, L.E. Mortenson, N.-H. Xuong, Proc. Natl. Acad. Sci. USA, 90, 1078 (1993).Google Scholar
5. Orme-Johnson, W.H., Science, 257, 1639 (1992); D. Sellmann, Ang. Chem. Int. Ed. Engl. 32, 54 (1993).Google Scholar
6. Thorneley, R.N.F. and Lowe, D.J., Biochem. J. 224, 887 (1984).Google Scholar
7. Conradson, S.D., Burgess, B.K., Newton, W.E., Mortenson, L.E., and Hodgson, K.O, J. Am. Chem. Soc. 109, 7507 (1987).Google Scholar
8. Arber, J.M., Flood, A.C., Garner, C.D., Gormal, C.A., Hasnain, S.S., and Smith, B.E., Biochem. J., 252, 421 (1988).Google Scholar
9. Chen, J. et. al. J. Am. Chem. Soc. (in press).Google Scholar
10. Rehr, J. J., Albers, R. C., Phys. Rev. B 41, 8139, (1991); J. J. Rehr, J. Mustree de Leon, S. I. Zabinsky, R. C. Albers, J. Am. Chem. Soc. 113, 5153 (1991).Google Scholar
11. Hales, B. J., Langosch, D. J., E. E.Case, J. Bio. Chem. 261, 15301 (1986).Google Scholar
12. Gurbiel, R. J., Bolin, J. T., Ronco, A. E., Mortenson, L., Hoffman, B. M., J. Magnetic Resonance, 91, 227 (1991).Google Scholar
13. Cramer, S. P., Tench, O., Yocum, M., George, G. N., Nucl. Inst. Meth. A 266, 586 (1988); S.P. Cramer, J. Chen, S.J. George, J. van Elp, J. Moore, O. Tensch, J. Colaresi, M. Yocum, O.C. Mullins, and C.T. Chen, Nucl. Instr. and Meth. A319, 285 (1992).Google Scholar
14. Flank, A. M., Weininger, M., Mortenson, L. E., Cramer, S. P., J. Am. Chem. Soc. 108, 1049 (1986).Google Scholar
15. Bolin, J.T., Ronco, A.E., Mortenson, L.E., Morgan, T.V., Williamson, M., and Xuong, N.-H., in Nitrogen Fixation: Achievements and Objectives, Gresshoff, Roth, Stacey, Newton, eds. Chapman and Hall, New York (1990) p. 117.Google Scholar
16. Chen, J. et. al. in Molybdenum Enzymes, ACS Symposium Series, Stiefel, E., ed. (in press).Google Scholar
17. van der Laan, G., Zaanen, J., Sawatzky, G.A., Karnatak, R., and Esteva, J.M., Phys. Rev. B, 33, 4253 (1986).Google Scholar
18. Groot, F.M.F. de, Fuggle, J.C., Thole, B.T., Sawatzky, G.A., Phys. Rev. B. 42,5459 (1990).CrossRefGoogle Scholar
19. Thole, B.T., Cowan, R.D., Sawatzky, G.A., Fink, J., Fuggle, J.C., Phys. Rev. B. 31, 6856 (1985).CrossRefGoogle Scholar
20. de Groot, F.M.F., Fuggle, J.C., Thole, B.T., Sawatzky, G.A., Phys. Rev. B. 41, 928 (1990).Google Scholar
21. George, S.J., van Elp, J., Chen, J., Ma, Y., Chen, C.T., Park, J.B., Adams, M.W.W., Searle, B.G., de Groot, F.M.F., Fuggle, J.C., and Cramer, S.P., Amer. Chem. Soc., 114, 442 (1992).Google Scholar
22. Chen, C.T. and Sette, F., Rev. Sci. Instr. 60, 1616 (1989); C.T. Chen, F. Sette, Y. Ma, S. Modesti, Phys. Rev. B, 42, 7262 (1990).CrossRefGoogle Scholar
23. Elp, J. van et. al. submitted to Proc. Natl. Acad. Sci.Google Scholar
24. Workshop Report, LBL-31854, 1991.Google Scholar