Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-13T00:44:20.476Z Has data issue: false hasContentIssue false
  • English
  • Français

Universal Biomolecule Binding Interlayers Created by Energetic Ion Bombardment

Published online by Cambridge University Press:  07 September 2011

Marcela M.M. Bilek ,
David R. McKenzie ,
Daniel V. Bax ,
Alexey Kondyurin ,
Yongbai Yin ,
Neil. J. Nosworthy ,
Stacey Hirsh ,
Keith Fisher  and
Anthony S. Weiss
Marcela M.M. Bilek
Affiliation:
School of Physics, University of Sydney, NSW 2006 Australia
David R. McKenzie
Affiliation:
School of Physics, University of Sydney, NSW 2006 Australia
Daniel V. Bax
Affiliation:
School of Physics, University of Sydney, NSW 2006 Australia School of Molecular Biosciences, University of Sydney NSW 2006 Australia
Alexey Kondyurin
Affiliation:
School of Physics, University of Sydney, NSW 2006 Australia
Yongbai Yin
Affiliation:
School of Physics, University of Sydney, NSW 2006 Australia
Neil. J. Nosworthy
Affiliation:
School of Physics, University of Sydney, NSW 2006 Australia School of Medical Sciences, University of Sydney, NSW 2006 Australia
Stacey Hirsh
Affiliation:
School of Physics, University of Sydney, NSW 2006 Australia
Keith Fisher
Affiliation:
School of Chemistry, University of Sydney, NSW 2006 Australia
Anthony S. Weiss
Affiliation:
School of Molecular Biosciences, University of Sydney NSW 2006 Australia
Get access

Abstract

The ability to strongly attach biomolecules such as enzymes and antibodies to surfaces underpins a host of technologies that are rapidly growing in utility and importance. Such technologies include biosensors for medical and environmental applications and protein or antibody diagnostic arrays for early disease detection. Emerging new applications include continuous flow reactors for enzymatic chemical, textile or biofuels processing and implantable biomaterials that interact with their host via an interfacial layer of active biomolecules. In many of these applications it is desirable to maintain physical properties of an underlying material whilst engineering a surface suitable for attachment of proteins or peptide constructs. Nanoscale polymeric interlayers are attractive for this purpose.

We have developed interlayers[1] that form the basis of a new biomolecule binding technology with significant advantages over other currently available methods. The interlayers, created by the ion implantation of polymer like surfaces, achieve covalent immobilization on immersion of the surface in protein solution. The interlayers can be created on any underlying material and ion stitched into its surface. The covalent immobilization of biomolecules from solution is achieved through the action of highly reactive free radicals in the interlayer.

In this paper, we present characterisation of the structure and properties of the interlayers and describe a detailed kinetic model for the covalent attachment of protein molecules directly from solution.

Keywords

biomaterialelectron spin resonanceion-implantation
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1354: Symposium II – Ion Beams—New Applications from Mesoscale to Nanoscale , 2011 , mrss11-1354-ii01-02
Copyright
Copyright © Materials Research Society 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

[1] Bilek, M. M. M., et al. , “Free radical functionalization of surfaces to prevent adverse responses to biomedical devices,” Proc. Nat. Acad. Sci., p. in press, 2011.10.1073/pnas.1103277108Google Scholar
[2] DeGrado, W. F., “Computational Biology – Biosensor Design,” Nature, vol. 423, pp. 132–133, 2003.10.1038/423132aGoogle Scholar
[3] Hanash, S., “Disease proteomics,” Nature, vol. 422, pp. 226–232, Mar 2003.10.1038/nature01514Google Scholar
[4] Olsson, P., et al. , “On the blood compatibility of end-point immobilized heparin,” J. Biomater. Sci. Polymer Edn. , vol. 11, pp. 1261–1273, 2000.10.1163/156856200744192Google Scholar
[5] Werner, C., et al. , “Current strategies towards hemocompatible coatings,” Journal of Materials Chemistry, vol. 17, pp. 3376–3384, 2007.10.1039/b703416bGoogle Scholar
[6] Nojiri, C., et al. , “Nonthrombogenic Polymer Vascular Prosthesis,” Artificial Organs, vol. 19, pp. 32–38, 1995.10.1111/j.1525-1594.1995.tb02241.xGoogle Scholar
[7] Paweletz, C. P., et al. , “Reverse phase protein microarrays which capture disease progression show activation of pro-survival pathways at the cancer invasion front,” Oncogene vol. 20, pp. 1981–1989, 2001.10.1038/sj.onc.1204265Google Scholar
[8] Pompe, W., et al. , “Functionally graded materials for biomedical applications,” Materials Science and Engineering A, vol. 362, pp. 40–60, 2003 10.1016/S0921-5093(03)00580-XGoogle Scholar
[9] Uchida, M., et al. , “Biomimetic coating of laminin-apatite composite on titanium metal and its excellent cell-adhesive properties,” Advanced Materials, vol. 16, pp. 1071–1074, 2004.10.1002/adma.200400152Google Scholar
[10] Bax, D. V., et al. , “The linker-free covalent attachment of collagen to plasma immersion ion implantation treated polytetrafluoroethylene and subsequent cell-binding activity,” Biomaterials, vol. 31, pp. 2526–2534, 2010.10.1016/j.biomaterials.2009.12.009Google Scholar
[11] Harman, D., “Aging: A Theory Based on Free Radical and Radiation Chemistry.,” J. Gerontol. , vol. 11, pp. 298–300, 1956.10.1093/geronj/11.3.298Google Scholar
[12] Giunta, S., et al. , “Transformation of beta-amyloid (AP) (1-42) tyrosine to L-Dopa as the result of in vitro hydroxyl radical attack,” Amvloid: Int. J. L h. Clin. Invest. , vol. 7, pp. 189–193, 2000.10.3109/13506120009146833Google Scholar
[13] Popok, V. N., et al. , “High fluence ion beam modification of polymer surfaces: EPR and XPS studies,” Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, vol. 178, pp. 305–310 2001.10.1016/S0168-583X(00)00491-2Google Scholar
[14] Jones, B. J., et al. , “Electron paramagnetic resonance study of ion implantation induced defects in amorphous hydrogenated carbon,” Diamond and Related Materials, vol. 10, pp. 993–997, 2001.10.1016/S0925-9635(00)00364-2Google Scholar
[15] Mesyats, G., et al. , “Adhesion of Polytetrafluorethylene modified by an ion beam,” Vacuum, vol. 52, pp. 285–289, 1999.10.1016/S0042-207X(98)00300-5Google Scholar
[16] Bilek, M. and McKenize, D. R., “Plasma modified surfaces for covalent immobilization of functional biomolecules in the absence of chemical linkers: towards better biosensors and a new generation of medical implants,” Biophysical Review, vol. 2, pp. 55–65, 2010.10.1007/s12551-010-0028-1Google Scholar
[17] Yin, Y. B., et al. , “Protein immobilization capacity and covalent binding coverage of pulsed plasma polymer surfaces,” Applied Surface Science, vol. 256, pp. 4984–4989, 2010.10.1016/j.apsusc.2010.03.013Google Scholar
[18] Yin, Y. B., et al. , “Acetylene plasma polymerized surfaces for covalent immobilization of dense bioactive protein monolayers,” Surface & Coatings Technology, vol. 203, pp. 1310–1316, 2009.10.1016/j.surfcoat.2008.10.035Google Scholar
[19] Yin, Y. B., et al. , “Covalently Bound Biomimetic Layers on Plasma Polymers with Graded Metallic Interfaces for in vivo Implants,” Plasma Processes and Polymers, vol. 6, pp. 658–666, 2009.10.1002/ppap.200900045Google Scholar
[20] Yin, Y. B., et al. , “Plasma Polymer Surfaces Compatible with a CMOS Process for Direct Covalent Enzyme Immobilization,” Plasma Processes and Polymers, vol. 6, pp. 68–75, 2009.10.1002/ppap.200800108Google Scholar
[21] Yin, Y. B., et al. , “Covalent immobilisation of tropoelastin on a plasma deposited interface for enhancement of endothelialisation on metal surfaces,” Biomaterials, vol. 30, pp. 1675–1681, 2009.10.1016/j.biomaterials.2008.11.009Google Scholar
[22] Yin, Y., et al. , “Acetylene plasma coated surfaces for covalent immobilization of proteins,” Thin Solid Films, vol. 517, pp. 5343–5346, 2009.10.1016/j.tsf.2009.03.045Google Scholar
[23] Nosworthy, N. J., et al. , “The attachment of catalase and poly-L-lysine to plasma immersion ion implantation-treated polyethylene,” Acta Biomaterialia, vol. 3, pp. 695–704, 2007.10.1016/j.actbio.2007.02.005Google Scholar
[24] MacDonald, C., et al. , “Covalent attachment of functional protein to polymer surfaces: a novel one-step dry process,” Journal of the Royal Society Interface, vol. 5, pp. 663–669, 2008.10.1098/rsif.2007.1352Google Scholar
[25] Kondyurin, A., et al. , “Attachment of horseradish peroxidase to polytetrafluorethylene (teflon) after plasma immersion ion implantation,” Acta Biomaterialia, vol. 4, pp. 1218–1225, 2008.10.1016/j.actbio.2008.04.017Google Scholar
[26] Kondyurin, A., et al. , “Covalent Attachment and Bioactivity of Horseradish Peroxidase on Plasma-Polymerized Hexane Coatings,” Plasma Processes and Polymers, vol. 5, pp. 727–736, 2008.10.1002/ppap.200800010Google Scholar
[27] Ho, J. P. Y., et al. , “Plasma-treated polyethylene surfaces for improved binding of active protein,” Plasma Processes and Polymers, vol. 4, pp. 583–590, 2007.10.1002/ppap.200600182Google Scholar
[28] Bax, D. V., et al. , “Linker-free covalent attachment of the extracellular matrix protein tropoelastin to a polymer surface for directed cell spreading,” Acta Biomaterialia, vol. 5, pp. 3371–3381, 2009.10.1016/j.actbio.2009.05.016Google Scholar
[29] Hirsh, S. L., et al. , “A Comparison of Covalent Immobilization and Physical Adsorption of a Cellulase Enzyme Mixture,” Langmuir, vol. 26, pp. 14380–14388., 2010.10.1021/la1019845Google Scholar
[30] Ganapathy, R., et al. , “Immobilization of papain on cold-plasma functionalized polyethylene and glass surfaces,” Journal of Biomaterial Science Polymer Edition vol. 12, pp. 1027–1049, 2001.10.1163/156856201753252543Google Scholar
[31] Kiaei, D., et al. , “Tight binding of albumin to glow discharge treated polymers,,” Journal of Biomaterial Science, Polymer Edition, vol. 4, pp. 35–44, 1992.10.1163/156856292X00286Google Scholar
[32] Bilek, M. M. M., et al. , “Free radical functionalization of surfaces to prevent adverse responses to biomedical devices,” PNAS, in press 2011.Google Scholar
[33] Wu, W. J., et al. , “Glycosaminoglycans mediate the coacervation of human tropoelastin through dominant charge interactions involving lysine side chains,” Journal of Biological Chemistry, vol. 274, pp. 21719–21724, Jul 1999.10.1074/jbc.274.31.21719Google Scholar
[34] Gan, B. K., et al. , “Etching and structural changes in nitrogen plasma immersion ion implanted polystyrene films,” Nuclear Instruments & Methods in Physics Research Section B-Beam Interactions with Materials and Atoms, vol. 247, pp. 254–260, 2006.Google Scholar
[35] Kondyurin, A., et al. , “Etching and structural changes of polystyrene films during plasma immersion ion implantation from argon plasma,” Nuclear Instruments & Methods in Physics Research Section B-Beam Interactions with Materials and Atoms, vol. 251, pp. 413–418, 2006.Google Scholar
[36] Jiang, H., et al. , “Surface oxygen in plasma polymerized films,,” Journal of Materials Chemistry, vol. 19, pp. 2234–2239, 2009.10.1039/b816814hGoogle Scholar