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Synthesis, Surface and Cell-Adhesion Properties of Polyurethanes Containing Covalently Grafted RGD-Peptides

Published online by Cambridge University Press:  15 February 2011

Horng-Ban Lin  and
Stuart L. Cooper
Horng-Ban Lin
Affiliation:
Meadox Medicals, Inc., 112 Bauer Drive, Oakland, NJ 07436
Stuart L. Cooper
Affiliation:
University of Delaware, College of Engineering, 135 DuPont Hall, Newark, DE 19716
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Abstract

In an attempt to improve endothelial cell adhesion and growth on a polyurethane copolymer, cell adhesive RGD-containing peptides were grafted to the polymer backbone. Two peptide grafting reaction schemes, including one-step and two-step approaches, were developed. Amino acid analysis confirmed that the two-step approach had a higher peptide coupling efficiency. The two-step reaction scheme was utilized to prepare GRGDSY, GRGDVY and GRGESY (inactive control) peptide grafted polyurethanes with two different peptide densities (100 and 250 μmol/g polymer). Dynamic contact angle measurements indicated that the surfaces of the peptide grafted polyurethanes were more hydrophilic than the starting and carboxylated versions of the precursor polyurethane. In-vitro endothelial cell adhesion experiments showed that, without the presence of serum in culture medium, the GRGDSY- and GRGDVY-grafted polyurethanes dramatically enhanced cell attachment and spreading. Increasing the peptide density from 100 to 250 μmol/g polymer for the GRGDSYand GRGDVY-grafted polyurethanes resulted in an increase in cell attachment. With approximately the same peptide density (100 or 250 μmol/g polymer), the GRGDVY-grafted polymers supported more adherent cells than the GRGDSY-grafted polymers. Similar trends were observed in the in-vitro endothelial cell growth studies using culture medium containing serum and endothelial cell growth supplement. These RGD-peptide grafted polyurethanes may be useful in providing an easily prepared cell-adhesive substrate for various implantable devices and hybrid organs.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 331: Symposium T – Biomaterials for Drug and Cell Delivery , 1993 , 105
Copyright
Copyright © Materials Research Society 1994

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