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Surface Interaction of Inflammatory Species with Titanium and Titanium Oxide

Published online by Cambridge University Press:  15 February 2011

R. Suzuki ,
G. Hirata ,
J. McKittrick  and
J.A. Frangos
R. Suzuki
Affiliation:
Bioengineering Department, University of California-San Diego, La Jolla, CA 92093
G. Hirata
Affiliation:
AMES, University of California-San Diego, La Jolla, CA 92093
J. McKittrick
Affiliation:
AMES, University of California-San Diego, La Jolla, CA 92093
J.A. Frangos
Affiliation:
Bioengineering Department, University of California-San Diego, La Jolla, CA 92093
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Abstract

Titanium has been successfully used for decades in dental and orthopedic implants, but the mechanism for this metal's biocompatible properties have not been determined. Our hypothesis is that this biocompatibility involves interaction between the surface layer of titanium dioxide on the metal implant and reactive oxygen mediators of the inflammatory response. The affect of different titanium surface oxide layers on the reactive oxygen mediators produced during the inflammatory response has never been examined. Peroxynitrite is a highly reactive and unstable compound produced in vivo by the reaction of nitric oxide with superoxide. We investigated if titanium oxides affect the stability of peroxynitrite by promoting its breakdown. Peroxynitrite levels can be measured by its absorbance at 302 nm. At pH= 13.2, we found a 100% increase in the rate of degradation of peroxynitrite in the presence of titanium particles. Peroxynitrite is capable of nitrating 4-hydroxyphenolacetic acid (4-HPA). The nitrated form of 4-HPA can be measured by its absorbance at 432nm. 3-morpholinosydnonimine (SIN-1), a nitric oxide donor, has been shown to produce superoxide during its breakdown resulting in the formation of peroxynitrite. At physiological pH (7.4), a solution of 0.5mM 4-HPA was exposed to 5mM SIN- 1 on passivated titanium surfaces. There was a decrease of 58% nitrated 4-HPA in the solution exposed to passivated titanium compared to controls. Unpassivated titanium surfaces resulted in only a 10% decrease of nitrated 4-HPA while titanium treated with hydrogen peroxide resulted in a 70% decrease in nitrated 4-HPA concentrations compared to controls. Zirconium and palladium were also tested. These experiments suggest that titanium is capable of enhancing the breakdown of the inflammatory compound peroxynitrite which may account for the metal's biocompatible properties.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 530: Symposium CC – Biomaterials Regulating Cell Function & Tissue Development , 1998 , 111
Copyright
Copyright © Materials Research Society 1998

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References

1 Bothe, R., Beaton, L.E. & Davenport, H.A. Surgery, Gynecology and Obstetrics 71, pp. 598–602 (1940).Google Scholar
2 Laing, P.G., Ferguson, A.B.J. & Hodge, E.S. Journal of Biomedical Materials Research 1, pp. 135–49 (1967).Google Scholar
3 Albrektsson, T., Branemark, P.I., Hansson, H.A., Kasemo, B., Larsson, K., Lundstrom, I., McQueen, D.H. & Skalak, R. Annals of Biomedical Engineering 11, pp. 1–27 (1983).Google Scholar
4 Mild, E.E. & Bannon, B.P. in Titanium Alloys in Surgical Implants ed. Luckey, H.A., Kubli, F. (ASTM, Philadelphia, 1981) p. 7–15.Google Scholar
5 Brown, S.A. & Lemons, J.E. Medical Applications of Titanium and Its Alloys: The Material and Biological Issues (ASTM, West Conshohocken, PA, 1996)Google Scholar
6 Kasemo, B. & Lausmaa, J. in The Bone-Biomaterial Interface ed. Davies, J.E. (University of Toronto Press, Toronto, 1991) p. 19–32.Google Scholar
7 Anderson, J. & Miller, K. Biomaterials 5, pp. 5–10 (1984).Google Scholar
8 Thomsen, P. & Ericson, L.E. in The Bone-Biomaterial Interface ed. Davies, J.E. (University of Toronto Press, Toronto, 1991) p. 153–164.Google Scholar
9 Huie, R.E. & Padmaja, S. Free Rad. Res. Comms. 18, pp. 195–199 (1993).Google Scholar
10 Miller, M.J., Thompson, J.H., Zhang, X.J., Sadowska-Krowicka, H., Kakkis, J.L., Munshi, U.K., Sandoval, M., Rossi, J.L., Eloby-Childress, S., Beckman, J.S. & et al. Gastroenterology 109, pp. 1475–83 (1995).Google Scholar
11 Rachmilewitz, D., Stamler, J.S., Karmeli, F., Mullins, M.E., Singel, D.J., Loscalzo, J., Xavier, R.J. & Podolsky, D.K. Gastroenterology 105, pp. 1681–8 (1993).Google Scholar
12 Royall, J.A., Kooy, N.W. & Beckman, J.S. New Horizons 3, pp. 113–122 (1995).Google Scholar
13 Crow, J.F. & Beckman, J.S. Advances in Pharmacology 34, pp. 17–43 (1995).Google Scholar
14 Koppenol, W.H., Moreno, J.J., Pryor, W.A., Ischiropoulos, H. & Beckman, J.S. Chemical Research in Toxicology v.5, pp. 834–842. (1992).Google Scholar
15 Szabo, C. Shock 6, pp. 79–88 (1996).Google Scholar
16 Williams, D. in Fundamental Aspects of Biocompatiblity ed. Williams, D. (CRC Press, Boca Raton, 1981) p. 11–42.Google Scholar
17 Effah, E., Bianco, P. & Ducheyne, P. Journal of Biomedical Materials Research 29, pp. 73–80 (1995).Google Scholar
18 Sundgren, J.E., Bodo, P. & Lundstrom, I. Journal of Colloid and Interface Science 110, pp. 9–20 (1986).Google Scholar
19 Tengvall, P., Elwing, H. & Lundstrom, I. Journal of Colloid and Interface Science 130, pp. 405–413 (1989).Google Scholar
20 Tengvall, P., Lundstrom, I., Sjoqvist, L., Elwing, H. & Bjursten, L. Biomaterials 10, pp. 166–75 (1989).Google Scholar
21 Tengvall, P., Walivaara, B., Westerling, J. & Lundstrom, I. Journal of Colloid and Interface Science 143, pp. 589–592 (1991).Google Scholar
22 Tengvall, P., Elwing, H., Sjoqvist, L., Lundstrom, I. & Bjursten, L. Biomaterials 10, pp. 118–20 (1989).Google Scholar
23 Pan, J., Thierry, D. & Leygraf, C. J Biomed Mater Res 28, pp. 113–22 (1994).Google Scholar
24 Beckman, J.S., Beckman, T.W., Chen, J., Marshall, P.A. & Freeman, B.A. Proceedings of the National Academy of Sciences of the United States of America v.87, pp. 1620–1624. (1990).Google Scholar
25 Feelisch, M. Journal of Cardiovascular Pharmacology 17, pp. S25–S33 (1991).Google Scholar
26 Beckman, J.S., Chen, J., Ischiropoulos, H. & Crow, J.P. Methods in Enzymology 233, pp. 229–241 (1994).Google Scholar
27 Crow, J.P., Beckman, J.S. & McCord, J.M. Biochemistry 34, pp. 3544–52 (1995).Google Scholar
28 Beckman, J.S., Ischiropoulos, H., Zhu, L. Van der, Woerd, M., Smith, C., Chen, J., Harrison, J., Martin, J.C. & Tsai, M. Archives of Biochemistry and Biophysics v.298, pp. 438–445. (1992).Google Scholar
29 Nair, M., Luo, Z.H. & Heller, A. Industrial & Engineering Chemistry Research 32, pp. 2318–2323 (1993).Google Scholar