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Speciation of Uranium After Microbial Action by XANES and XPS

Published online by Cambridge University Press:  15 February 2011

Cleveland J. Dodge ,
A. J. Francis ,
F. Lu ,
G. P. Halada ,
S. V. Kagwde  and
C. R. Clayton
Cleveland J. Dodge
Affiliation:
Department of Applied Science, Brookhaven National Laboratory, Upton, NY 11973
A. J. Francis
Affiliation:
Department of Applied Science, Brookhaven National Laboratory, Upton, NY 11973
F. Lu
Affiliation:
Department of Applied Science, Brookhaven National Laboratory, Upton, NY 11973
G. P. Halada
Affiliation:
Department of Materials Science, State University of New York at Stony Brook, Stony Brook, NY 11794
S. V. Kagwde
Affiliation:
Department of Materials Science, State University of New York at Stony Brook, Stony Brook, NY 11794
C. R. Clayton
Affiliation:
Department of Materials Science, State University of New York at Stony Brook, Stony Brook, NY 11794
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Abstract

The speciation of radionuclides and toxic metals in wastes subjected to microbial action is important in determining the extent of stabilization in a disposal environment. As part of an ongoing study, we investigated the reduction of uranium by a Clostridium sp. using X-ray absorption near edge spectroscopy (XANES) at the National Synchrotron Light Source (NSLS) and X-ray photoelectron spectroscopy (XPS). XPS analysis of uranyl acetate containing hexavalent uranium exhibited a binding energy of 382.0eV at the U 4f7/2 peak. The sample incubated in the presence of bacteria was shifted to lower binding energy (380.6eV), confirming the reduction of U6+ to U4+ at the bacterial surface. XANES analysis, using an electron yield detector, was performed at the Mv absorption edge (3d--> 5f). The absorption peak energy of the sample exhibited a shift from 3551.1eV to 3550.1eV which is higher than uranium metal (3549.6eV) but lower than U4+ (3550.4eV). This indicates the presence of U3+ which is probably located beneath the surface within the biomass. Anaerobic bacterial treatment of wastes containing uranyl ion can result in the stabilization of uranium.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 307: Symposium L – Applications of Synchrotron Radiation Techniques to Materials Science , 1993 , 89
Copyright
Copyright © Materials Research Society 1993

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References

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