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Smart Surfaces: Use of Electrokinetics for Selective Modulation of Biomolecular Affinities

Published online by Cambridge University Press:  24 January 2012

Sam Emaminejad ,
Mehdi Javanmard ,
Robert W. Dutton  and
Ronald W. Davis
Sam Emaminejad
Affiliation:
Stanford University, Stanford, California, U.S.A Stanford Genome Technology Center, Stanford, California, U.S.A
Mehdi Javanmard
Affiliation:
Stanford Genome Technology Center, Stanford, California, U.S.A
Robert W. Dutton
Affiliation:
Stanford University, Stanford, California, U.S.A
Ronald W. Davis
Affiliation:
Stanford Genome Technology Center, Stanford, California, U.S.A
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Abstract

With the aid of negative dielectrophoresis (nDEP) force in conjunction with shear force and at an optimal sodium hydroxide (NaOH) concentration we demonstrated a switch-like functionality to elute immuno-bound beads from the surface. At an optimal flow rate and NaOH concentration, nDEP turned on results in bead detachment, whereas when nDEP is off, the beads remain attached. This platform offers the potential for performing a bead-based multiplexed immunoassay where in a single channel various regions are immobilized with a different antibody, each targeting a different antigen. As a proof of concept we demonstrated the ability of nDEP to provide this switching behavior in a singleplex assay for the interactions that were in the same order of magnitude in strength as typical antibody-antigen interactions.

Keywords

biomedicalkineticsfluidics
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1415: Symposium HH/II/TT – MEMS, BioMEMS and Bioelectronics–Materials and Devices , 2012 , mrsf11-1415-ii08-07
Copyright
Copyright © Materials Research Society 2012

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References

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