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Single Cell Based Microelectrode Array Biosensors

Published online by Cambridge University Press:  15 February 2011

Mo Yang ,
Shalini Prasad ,
Xuan Zhang ,
Mihrimah Ozkan  and
Cengiz S. Ozkan
Mo Yang
Affiliation:
Department of Mechanical Engineering
Shalini Prasad
Affiliation:
Department of Electrical Engineering
Xuan Zhang
Affiliation:
Department of Mechanical Engineering
Mihrimah Ozkan
Affiliation:
Department of Electrical Engineering Department of Chemical and Environmental Engineering University of California, Riverside, CA 92521
Cengiz S. Ozkan
Affiliation:
Department of Mechanical Engineering
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Abstract

Extracellular potential is an important parameter which indicates the electrical activity of live cells. Membrane excitability in osteoblasts plays a key role in modulating the electrical activity in the presence of chemical agents. The complexity of cell signal makes interpretation of the cellular response to a chemical agent very difficult. By analyzing shifts in the signal power spectrum, it is possible to determine a frequency spectrum also known as Signature Pattern Vectors (SPV) specific to a chemical. It is also essential to characterize single cell sensitivity and response time for specific chemical agents for developing detect-to-warn biosensors. We used a 4x4 multiple Pt microelectrode array to spatially position single osteoblast cells, by using a gradient AC field. Fast Fourier Transformation (FFT) and Wavelet Transformation (WT) analyses were used to extract information pertaining to the frequency of firing from the extracellular potential.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 773: Symposium N – Biomicroelectromechanical Systems (BioMEMS) , 2003 , N11.6
Copyright
Copyright © Materials Research Society 2003

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References

1. Lavrik, N. V., Tipple, C. A., Sepaniak, M. J. and Datskos, D., Gold Nano-Structure for Transduction of Biomolecular Interactions into Micrometer Scale Movements, Biomedical Microdevices, 3:1, 2001, 35–44.Google Scholar
2. Lu, P., Shen, F., O'shea, S. J., Lee, K.H. and Ng, T.Y., Analysis of Surface Effects on Mechanical Properties of Microcantilevers, Mater. Phys. Mench. 4, 2001, 51–55.Google Scholar
3. Moulin, A. M., O'Shea, S. J. and Welland, M. E., Microcantilever-based Biosensors, Ultramicroscopy, 82, 2000, 23–31.Google Scholar
4. Piezoelectric Technology Data for Designers, Morgan Matroc Inc., Electro Ceramics Division., 2000, p.14.Google Scholar
6. Pritchard, W. F., Davis, P. F., Derafshi, Z., Polacek, D. C., Tsoa, R., Dull, R. O., Jones, S. A. and Giddens, D. P., Effects of Wall Shear Stress and Fluid Recirculation on the Localization of circulating Monocytes in a Three Dimensional flow model, J. of Biomechanics, 28, 1995, 1459–1469.Google Scholar
7. Raiteri, R. and Nelles, G., Butt, H. J., Knoll, W. and Skladal, P., Sensing of Biological Substances Based on the Bending of the Microfabricated Cantilevers, Sensors and Actuators B, 61, 1999, 213–217.Google Scholar
8. Ramakrishnan, A. and Sadana, A., A Predictive Approach using Fractal Analysis for Analyte-Receptor Binding and Dissociation Kinetics for Surface Plasmon Resonance Biosensor Applications J. of interface and Colloid Science, 229, 2000, 628–640.Google Scholar
10. Ulman, A, Formation and Structure of Self Asswmbled Monolayers, Chem. Rev., 96, 1996, 1533–1554.Google Scholar
11. Vo-Dinh, T., Cullum, B. M., Stokes, D. L., Nanosensors and Biochips: Frontiers in Biomolecular Diagnostics, Sensors and Actuators B, 74, 2001, 2–11.Google Scholar
12. Wu, G., Ji, H., Hansen, K., Thundat, T., Datar, R., Cote, R., Hagan, M. F., Chakraborty, A. K., and Majumdar, A., Origin of Nanomechanical Cantilever Motion Generated from Biomolecular Interactions, PNAS, 98, 2001, 1560–1564.Google Scholar
13 Khaled, A. R. A., Vafai, K., Ozkan, C. S., Yang, M. and Zhang, X., “Analysis, Control and Augmentation of Microcantilever Deflections in Bio-Sensing Systems”, Journal of Sensors and Actuators, B (submitted).Google Scholar
14. Yang, Mo, Zhang, Xuan and Ozkan, Cengiz S., “Modeling and Optimal Design of Piezoresistive Cantilevers for Biosensing Applications” Journal of Biomedical Microdevices (submitted).Google Scholar