Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-29T06:56:14.605Z Has data issue: false hasContentIssue false
  • English
  • Français

Simultaneous gene delivery and tracking of cells using fluorescent upconversion nanoparticles for cell therapy

Published online by Cambridge University Press:  25 July 2011

Muthu Kumara Gnanasammandhan Jayakumar  and
Yong Zhang
Muthu Kumara Gnanasammandhan Jayakumar
Affiliation:
Division of Bioengineering, Faculty of Engineering, National University of Singapore, 117576, Singapore
Yong Zhang*
Affiliation:
Division of Bioengineering, Faculty of Engineering, National University of Singapore, 117576, Singapore Nanoscience and Nanotechnology Initiative, National University of Singapore, 117576, Singapore
*
*E-mail: [email protected].
Get access

Abstract

Cell therapy has attained new heights with the current developments in the field of stem cell therapy and is often done in combination with gene therapy. Much research has gone into developing safe vectors to deliver genes to the cells used for cell therapy and also in tracking the transplanted cells and determining their fate. Even though viral vectors are very efficient in gene delivery, there are major safety concerns related to it. Herein, we report the use of novel fluorescent upconversion nanoparticles (UCNs) for simultaneous gene delivery and tracking of cells for cell-based therapies. It is a safe alternative to viral vectors and offers imaging capabilities too, which will give an insight on the fate of transplanted cells.

Keywords

biomedicalluminescencenanostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1355: Symposium JJ – Biological Hybrid Materials for Life Sciences , 2011 , mrss11-1355-jj07-02
Copyright
Copyright © Materials Research Society 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1 Sugaya, K., Brannen, C. L., Medical hypotheses 2001, 57, 697.10.1054/mehy.2001.1424Google Scholar
2 Fan, H., Liu, H., Wong, E. J. W., Toh, S. L., Goh, J. C. H., Biomaterials 2008, 29, 3324.10.1016/j.biomaterials.2008.04.012Google Scholar
3 Domínguez-Bendala, J., Klein, D., Ribeiro, M., Ricordi, C., Inverardi, L., Pastori, R., Edlund, H., Diabetes 2005, 54, 720.10.2337/diabetes.54.3.720Google Scholar
4 Chatterjee, D. K., Rufaihah, A. J., Zhang, Y., Biomaterials 2008, 29, 937.10.1016/j.biomaterials.2007.10.051Google Scholar
5 Wang, M., Mi, C. C., Wang, W. X., Liu, C. H., Wu, Y. F., Xu, Z. R., Mao, C. B., Xu, S. K., ACS Nano 2009, 3, 1580.10.1021/nn900491jGoogle Scholar
6 Qian, H. S., Guo, H. C., Ho, P. C., Mahendran, R., Zhang, Y., Small 2009, 5, 2285.10.1002/smll.200900692Google Scholar