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Selective Attachment of Synthetic DNA to Self-Assembled-Monolayer Functionalized Surfaces

Published online by Cambridge University Press:  15 February 2011

Linda A. Chrisey ,
Paul M. Roberts ,
Valarie I. Benezra ,
Walter J. Dressick ,
Charles S. Dulcey  and
Jeffrey M. Calvert
Linda A. Chrisey
Affiliation:
Naval Research Laboratory, Center for Bio/Molecular Science and Engineering (Code 6900), 4555 Overlook Ave SW, Washington, DC 20375-5348
Paul M. Roberts
Affiliation:
Geo-Centers, Inc., Fort Washington, MD 20744
Valarie I. Benezra
Affiliation:
Geo-Centers, Inc., Fort Washington, MD 20744
Walter J. Dressick
Affiliation:
Naval Research Laboratory, Center for Bio/Molecular Science and Engineering (Code 6900), 4555 Overlook Ave SW, Washington, DC 20375-5348
Charles S. Dulcey
Affiliation:
Naval Research Laboratory, Center for Bio/Molecular Science and Engineering (Code 6900), 4555 Overlook Ave SW, Washington, DC 20375-5348
Jeffrey M. Calvert
Affiliation:
Naval Research Laboratory, Center for Bio/Molecular Science and Engineering (Code 6900), 4555 Overlook Ave SW, Washington, DC 20375-5348
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Abstract

Self-assembled monolayer (SAM) films of organosilanes have been characterized as surfaces for the spatially-controlled, selective attachment of single-stranded synthetic DNA. Geometric patterns of DNA on silica substrates have been created by photochemical modification of the SAMs to produce regions which promote or resist DNA attachment. The attachment of DNA to the modified surfaces was followed using UV spectroscopy, radioassay, ellipsometry, and enzyme-linked colorimetric assay. We have also demonstrated that immobilized DNA is capable of hybridization with a complementary strand under stringent conditions.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 330: Symposium S – Biomolecular Materials by Design , 1993 , 179
Copyright
Copyright © Materials Research Society 1994

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References

REFERENCES

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