Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-23T14:26:15.562Z Has data issue: false hasContentIssue false
  • English
  • Français

A Sarcomere-Mimetic Gel: Gelation of Astral-Shaped Actin Filaments with Their Plus End Connected on Photopolymer Beads by Myosin Filaments

Published online by Cambridge University Press:  12 March 2013

Taiji Ikawa ,
Masahito Shiozawa ,
Makoto Mouri ,
Mamiko Narita  and
Osamu Watanabe
Taiji Ikawa
Affiliation:
Toyota Central R&D Labs., Inc., Nagakute, Aichi, 480-1192, Japan
Masahito Shiozawa
Affiliation:
Toyota Central R&D Labs., Inc., Nagakute, Aichi, 480-1192, Japan
Makoto Mouri
Affiliation:
Toyota Central R&D Labs., Inc., Nagakute, Aichi, 480-1192, Japan
Mamiko Narita
Affiliation:
Toyota Central R&D Labs., Inc., Nagakute, Aichi, 480-1192, Japan
Osamu Watanabe
Affiliation:
Toyota Central R&D Labs., Inc., Nagakute, Aichi, 480-1192, Japan
Get access

Abstract

We have developed a method of a stepwise construction of a gel consisting of (i) astral-shaped actin filaments with their plus end connected on photo-responsive polymer beads and (ii) bipolar myosin filaments as linkers in order to mimic sarcomeric structure, the basic unit of a muscle. In the method, firstly, 4 μm diam. beads were prepared from an acrylate polymer containing azobenzene moiety by a good-solvent evaporation technique. Next, gelsolin, which servers and remains bound to the plus end of an actin filament, was adsorbed and then immobilized on the bead surface by exposure to light from blue light-emitting diodes, and then fluorescent actin filaments were mixed with the beads. Formation of star-like, astral actin filaments on the beads were observed in fluorescent microscopy. Finally, the beads with actin filaments were mixed with myosin mini filaments with ca. 1 μm in length. Dozens of the beads were observed to be assembled into a gel form in optical microscopy. After adding adenosine triphosphate to the gel solution, the gel was slowly contract up to 60% comparing with its original volume, suggesting that linker myosin filaments moved on the actin filaments toward the plus end on the beads.

Keywords

biomimetic (assembly)biomaterialnanostructure
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1498: Symposium Q – Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications , 2013 , pp. 213 - 218
Copyright
Copyright © Materials Research Society 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K. and Walter, P. in Moleuclar Biology of the Cell, forth edition (Garland Science, 2002) pp. 949–968.Google Scholar
Kron, S. J., Spudich, J. A., Proc. Natl. Acad. Sci. USA. 83, 6272–6276 (1986).CrossRefGoogle Scholar
Toyoshima, Y. Y., Kron, S. J.. McNally, E. M., Niebling, K. R., Toyoshima, C., Spudich, J. A., Nature, 328, 536–539 (1987).CrossRefGoogle Scholar
Hiratsuka, Y., Tada, T., Oiwa, K., Kanayama, T., Uyeda, T. Q. P., Biophys. J., 81, 1555–1561 (2001).CrossRefGoogle Scholar
Ikawa, T., Hoshino, F., Matsuyama, T., Takahashi, H., and Watanabe, O., Langmuir 22, 2747 (2006).CrossRefGoogle Scholar
Ikawa, T., Kato, Y., Yamada, T., Shiozawa, M., Mouri, M. M., Hoshino, F., Watanabe, O., Tawata, M., Shimoyama, H., Langmuir, 26, 12673–12679 (2010).CrossRefGoogle Scholar
Yabu, H., Higuchi, T., Ijiro, K. and Shimomura, M., Chaos, 15, 047505 (2005).CrossRefGoogle Scholar
Wong, G. C. L., Lin, A., Tang, J. X., Li, Y., Janmey, P.A., Safinya, C. R., Phys. Rev. Lett., 91 08103 (2003).CrossRefGoogle Scholar
Offer, G., Chapter 12 Myosin filaments, in Fibrous Protein Structure (Academic Press, 1987) pp. 307–356.Google Scholar