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Remineralization of Artificial Dentin Lesions via the Polymer-Induced Liquid-Precursor (PILP) Process

Published online by Cambridge University Press:  13 July 2011

Taili Thula-Mata ,
Anora Burwell ,
Laurie B. Gower ,
Stefan Habelizt  and
Grayson Marshall
Taili Thula-Mata
Affiliation:
Materials Science & Engineering Department, University of Florida, Gainesville, FL 32611, U.S.A.
Anora Burwell
Affiliation:
Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, CA, U.S.A.
Laurie B. Gower
Affiliation:
Materials Science & Engineering Department, University of Florida, Gainesville, FL 32611, U.S.A.
Stefan Habelizt
Affiliation:
Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, CA, U.S.A.
Grayson Marshall
Affiliation:
Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, CA, U.S.A.
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Abstract

Acid-etched dentin samples with a zone of demineralized dentin were remineralized via the polymer-induced liquid-precursor (PILP) process. Poly-L-aspartic acid was used as the polymeric process-directing agent. Samples were incubated in the mineralization solution for 1- 4 weeks. Dentin samples remineralized by the PILP process presented a surface morphology very similar to the intact mineralized dentin’s architecture, in contrast to samples mineralized via the conventional nucleation and growth method (without polymer additive), which led to a superficial crust of randomly organized mineral crystals. Energy dispersive x-ray spectroscopy analysis of the PILP-mineralized samples showed the presence of calcium and phosphate ions at high levels. Since no hydroxyapatite (HA) clusters were observed on the surface of the PILP-mineralized samples, we could conclude the signal was produced from the mineral embedded within the dentin matrix. TEM and diffraction analyses suggest that both intrafibrillar and interfibrillar remineralization occurred in the demineralized dentin matrix.

Keywords

amorphousbiomimetic (chemical reaction)crystal growth
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1355: Symposium JJ – Biological Hybrid Materials for Life Sciences , 2011 , mrss11-1355-jj08-01
Copyright
Copyright © Materials Research Society2011

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References

REFERENCES

1. Gower, L.B. and Odom, D.J.. J. Crystal Growth, 210, 719 (2000).10.1016/S0022-0248(99)00749-6Google Scholar
2. Dai, L., Douglas, E.P. and Gower, L.B.. Journal of Non-Crystalline Solids, 354, 1845 (2008).10.1016/j.jnoncrysol.2007.10.022Google Scholar
3. Olszta, M.J., Cheng, X., Jee, S.S., Kumar, R., Kim, Y-Y, Kaufman MJ, M.J., et al. Materials Science & Engineering R-Reports 2007 July 20, 58, 77 (2007).Google Scholar
4. Jee, SS, Thula, TT, Gower, LB. Development of bone-like composites via the polymer-induced liquid-precursor (PILP) process: 1) Influence of polymer molecular weight. Acta Biomaterialia;2010;6:3676–3686.Google Scholar
5. Thula, T.T., Svedlund, F., Rodriguez, D.E., Podschun, J., Pendi, L., Gower, L.B.. Polymers, 3, 10 (2011).10.3390/polym3010010Google Scholar
6. Weiner S, S. and Wagner, H.D.. Annu. Rev. Mater. Sci. 28, 271 (1998).10.1146/annurev.matsci.28.1.271Google Scholar
7. Jee, S.S, Kasinath, R.K, DiMasi, E., Kim, Y.Y., Gower, L.B.. CrystEngComm, 13, 2077 (2011).10.1039/c0ce00605jGoogle Scholar
8. LeGeros, R.Z.. Calcium Phosphate in Oral Biology and Medicine. N.Y.: Karger, (1991).Google Scholar
9. Dorozhkin, S.V.. Materials 2, 399 (2009).10.3390/ma2020399Google Scholar
10. Lowenstam, H.A. and Weiner, S.. On Biomineralization. N.Y.: Oxford University Press, (1989).Google Scholar
11. Glimcher, M.J., in The nature of the mineral phase in bone: Biological and clinical implications, edited by Avioli, L.V. and Krane, S.M.. Metabolic bone disease and clinically related disorders (Academic Press, San Diego, CA, 1998) pp. 23–50.Google Scholar
12. Kikuchi, M., Itoh, S., Ichinose, S., Shinomiya, K., Tanaka, J.. Biomaterials 22, 1705 (2001).10.1016/S0142-9612(00)00305-7Google Scholar
13. Murugan, R., Ramakrishna, S.. Crystal Growth & Design 5, 111 (2005).10.1021/cg034227sGoogle Scholar
14. Nalla, R.K., Portera, A.E., Daraio, C., Minor, A.M., Radmilovic, V., Stach, E.A., Tomsia, A.P., Ritchie, R.O.. Micron 36, 672 (2005).10.1016/j.micron.2005.05.011Google Scholar
15. Liu, Y., Kim, Y-K., Dai, L., Li, N., Khan, S.O., Pashley, D.H., Tay, F.R.. Biomaterials 32, 1291 (2011).10.1016/j.biomaterials.2010.10.018Google Scholar
16. Tay, F.R. and Pashley, D.H.. Biomaterials, 29, 1127 (2007).10.1016/j.biomaterials.2007.11.001Google Scholar