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Raman Spectrum Modification of CdSe/ZnS Quantum Dots at the Bio-conjugation to IgG Antibodies

Published online by Cambridge University Press:  08 February 2012

A. I. Diaz Cano ,
J. Douda ,
C. R. Gonzalez Vargas  and
K. Gazarian
A. I. Diaz Cano
Affiliation:
UPIITA – Instituto Politécnico Nacional, México D. F. 07738, México.
J. Douda
Affiliation:
UPIITA – Instituto Politécnico Nacional, México D. F. 07738, México.
C. R. Gonzalez Vargas
Affiliation:
UPIITA – Instituto Politécnico Nacional, México D. F. 07738, México.
K. Gazarian
Affiliation:
Institute of Biomedical Investigations at UNAM, Mexico D.F. Mexico.
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Abstract

The paper presents the analysis of Raman scattering spectra of non-conjugated and bio-conjugated CdSe/ZnS core–shell quantum dots in the range of Raman shifts of 80 - 2000 cm-1. Commercial CdSe/ZnS QDs covered by polymer and characterized by color emission with the maxima at 605 and 655 nm (1.89 and 2.04 eV) were used. Raman scattering spectra were measured at 300K and the excitation by the line 785.0 nm of a solid state LED. The analysis of Raman spectra has shown that the QD bio-conjugation to the immunoglobulin G (IgG) antibodies of the Pseudorabies virus is accompanied by the changes of the intensity of Raman lines related to the CdSe/ZnS core/shell QDs, PEG polymer covered QDs, the Si substrate and/or some organic groups of antibody molecules. The comparison of Raman spectra of CdSe/ZnS QDs with different sizes in non-conjugated and bio-conjugated states gives the opportunity to detect the bio-conjugation without mistake.

Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1371: Symposium S1 – Nanostructured Materials and Nanotechnology , 2012 , imrc11-1371-s1-62
Copyright
Copyright © Materials Research Society 2012

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References

REFERENCES

1. Abazović, N. D., Kuljanin-Jakovljević, J. Ň. and Čomor, M. I., Russian J. Phys. Chem. A 83, 9 1511 (2009).Google Scholar
2. Zhang, B., Liang, X., Hao, L., Cheng, J., Gong, X., Liu, X., Ma, G., Chang, J., J. Photochem. & Photobiol. B., 94, 45 (2009).Google Scholar
3. Dabbousi, B.O., Rodriguez Viejo, J., Mikulec, F.V., Heine, J.R., Mattoussi, H., Ober, R., Jensen, K.F., Bawendi, M.G., J. Phys. Chem. B. 101, 9463 (1997).Google Scholar
4. Torchynska, T. V., Nanotechnology, 20, 095401 (2009)Google Scholar
5. Dubertret, B., Skourides, P., Norris, D. J., Nolreaux, V., Brivanlou, A. H., Libchaber, A., Science, 298, 1759, 2002.Google Scholar
6. Ji, X., Zheng, J., Xu, J., Rastogi, V. K., Cheng, T.C., DeFrank, J.J., Leblanc, R.M., J. Phys. Chem. B 109, 3793 (2005).Google Scholar
7. Dybiec, M., Chomokur, G., Ostapenko, S., Wolcott, A., Zhang, J. Z., Zajac, A., Phelan, C., Sellers, T., Gerion, G., Appl. Phys. Lett. 90, 263112 (2007).Google Scholar
8. Torchynska, T. V., Douda, J., Ostapenko, S. S., Jimenez-Sandoval, S., Phelan, C., Zajac, A., Zhukov, T., Sellers, T., J. of Non-Crystal. Solid. 354, 2885 (2008).Google Scholar
9. Torchynska, T. V., Diaz Cano, A., Dybic, M., Ostapenko, S., Morales Rodriguez, M., Jimenez Sandoval, S., Vorobiev, Y., Phelan, C., Zajac, A., Zhukov, T., Sellers, T., phys. stat. sol. (c), 4, 241 (2007).Google Scholar
10. www.invitrogen.com Google Scholar
11. Diaz Cano, A., Jimenez Sandoval, S., Vorobiev, Y., Rodriguez Melgarejo, F. and Torchynska, T. V., Nanotechnology, 21, 134016 (2010).Google Scholar
12. Torchynska, T. V., Diaz Cano, A., Dybiec, M., Ostapenko, S., Morales Rodrigez, M. Google Scholar
13. Jiménez-Sandoval, S., Vorobiev, Y., Phelan, C., Zajac, A., Zhukov, T., and Sellers, T., Phys. Stat. Sol. (c) 4, No. 2, 241 (2007).Google Scholar
14. Temple, P.A. and Hathaway, C. E., Phys. Rev. B, 7, 3685 (1973).Google Scholar
15. Johnson, F.A. and Loudon, R., Proc. Roy. Soc. A, 281, 274 (1964).Google Scholar
16. Vega Macotela, L. G., Torchynska, T. V., Douda, J., and Peña Sierra, R., Variation of Raman spectra of CdSe/ZnS quantum dots at the bioconjugation, phys.stat.solid. (c), 7, 1192 (2010).Google Scholar
17. Nakamoto, K., Infrared and Raman Spectra of Inorganic and Coordination Compounds, Part A, John Wiley &Sons, Inc.1997, 386.Google Scholar
18. Baranov, A. V., Rakovich, Yu. P., Donegan, J. F., Perova, T.S., Moore, R.A., Talapin, D. V., Rogach, A. L., Masumoto, Y., Nabiev, I., Phys. Rev. B, 68, 165306 (2003).Google Scholar
19. Vega Macotela, L. G., Douda, J., Torchynska, T. V., Peña Sierra, R. and Shcherbyna, L., phys.stat.solid. (c), 7, 724–727, 2010.Google Scholar
20. Barron, L.D., Buckingham, A.D., Chem. Phys. Lett. 492, 199 (2010).Google Scholar