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Protein Adsorption on Amorphous Metal Oxide Thin Films: An FTIR/ATR and Ellipsometry study

Published online by Cambridge University Press:  01 February 2011

Phaedra Silva-Bermudez  and
Sandra E. Rodil
Phaedra Silva-Bermudez
Affiliation:
Instituto de Investigaciones en Materiales, UNAM, Circuito Exterior s/n, C.U., 04510, México D.F. México. División de Estudios de Posgrado, Facultad de Odontología, UNAM, Circuito Exterior s/n, C.U., 04510, México D.F., México. [email protected]
Sandra E. Rodil
Affiliation:
Instituto de Investigaciones en Materiales, UNAM, Circuito Exterior s/n, C.U., 04510, México D.F. México.
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Abstract

The adsorption of bovine serum albumin (BSA) and fibrinogen proteins dissolved on Phosphate buffer solution onto Ta, Nb and Ti oxide thin films was studied. The metal oxide thin films were deposited by magnetron sputtering on Si(100) wafers and characterized by contact angle measurements and profilometry. Spectroscopic ellipsometry was employed to characterize the kinetics of the protein adsorption process in-situ at the solid-liquid interface and the optical properties of the adsorbed protein layer formed after 45 minutes of immersion of the thin film in the protein solution. Infrared spectroscopy was used to study the proteins within the adsorbed layer. A trend indicating that the surface mass density of the adsorbed protein layer increases as the Rt (peak-to-valley height) surface roughness parameter increases was observed for fibrinogen and BSA. An increment in the surface mass density of the adsorbed protein layer was also observed onto surfaces with higher polar components of the surface energy. BSA and fibrinogen seemed to more readily adsorbed onto tantalum oxide than onto titanium oxide.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1277: Biomaterials–2010 , 2010 , S6-6
Copyright
Copyright © Materials Research Society 2010

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References

1. Anselme, K., Biomateriales 21, 687–681 (2000).Google Scholar
2. Wilson, C. J., Clegg, R. E., Leavesley, D. I. and Pearcy, M. J., Tissue Eng. 11, 1–18 (2005).Google Scholar
3. Zangooie, S., Bjorklund, R. and Arwin, H., Thin Solid Films 313, 825–830 (1998).Google Scholar
4. Yang, Y., Cavin, R. and Ong, J. L., J. Biomed. Mater. Res. A 67 344–349 (2003).Google Scholar
5. Peters, T. J., All About Albumin: Biochemistry, Genetics and Medical Applications (Academic Press, San Diego, CA, 1996) pp. 9–75.Google Scholar
6. McClellan, S. J., Franses, E. I., Colloids and Surf. A: Physicochem. Eng. Aspects 260, 265–275 (2005).Google Scholar
7. Peters, T. J., in Advances in Protein Chemistry, edited by Anfinsen, C. B., Edsall, J. T. and Richards, F. M. (Academic Press Inc., 1985) Vol. 37, pp. 161–245.Google Scholar
8. Clarke, M. L., Wang, J. and Chen, Z., J. Phys. Chem. B 109, 22027–22035 (2005).Google Scholar
9. Feng, L. and Andrade, J. D. in Proteins at Interfaces II. Fundamentals and Applications, edited by Horbett, T.A. and Brash, J. L. (American Chemical Society, Washington D.C. 1995) pp. 66–79.Google Scholar
10. Mosesson, M.W., Siebenlist, K.R. and Meh, D.A., Ann. N. Y. Acad. Sci. 936, 11–30 (2001).Google Scholar
11. Tengvall, P., Lundstrom, I., Liedberg, B., Biomaterials 19, 407–422 (1998).Google Scholar
12. Arwin, H. in Handbook of ellipsometry, edited by HG, Tompkins (J K Wilkinson, 2005) pp. 800–854.Google Scholar
13. Liedberg, B., Ivarsson, B. and Lundstrom, I., J. Biochem. Biophys. Meth. 9, 233–243 (1984).Google Scholar
14. Militello, V., Casarino, C., Emanuele, A., Giostra, A., Pullara, F. and Leone, M., Biophys. Chem. 107, 175–187 (2004).Google Scholar
15. Bagno, A. and Bello, C. Di, J. Mater. Sci. Med. 15, 935–949 (2004).Google Scholar
16. Stout, K. J. and Blunt, L., Three Dimensional Topography, (Kingsley publishers; 1994).Google Scholar
17. Van Oss, C. J., J. Colloid. Surf. B: Biointerfaces 5, 91–110 (1995).Google Scholar
18. Martensson, J. and Arwin, H., Langmuir 11, 963–968 (1995).Google Scholar
19. berlind, T., Poksinski, M., Tengvall, P. and Arwin, H., Colloids and Surf. B: Biointerfaces 75, 410–417 (2010).Google Scholar
20. Haschemeyer, A. E. V., Biochemistry 2, 851–858 (1963).Google Scholar
21. Horbett, T. A., Cheng, C. M., Ratner, B. D., Hoffmen, A. S., Hanson, S. R., J. Biomed. Mater. Res. 20, 739–742 (1986).Google Scholar