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Proliferation of Human Osteoblastic Cells and Synthesis of Extracellular Bone Matrix on Biomaterials

Published online by Cambridge University Press:  15 February 2011

D. J. Schaefer ,
B. Munder ,
M. Voigt  and
G. B. Stark
D. J. Schaefer
Affiliation:
Department of Plastic and Handsurgery, Valley Tissue Engineering Center, Albert-Ludwigs-University, D-79106 Freiburg, [email protected]
B. Munder
Affiliation:
Department of Plastic and Handsurgery, Valley Tissue Engineering Center, Albert-Ludwigs-University, D-79106 Freiburg, Germany
M. Voigt
Affiliation:
Department of Plastic and Handsurgery, Valley Tissue Engineering Center, Albert-Ludwigs-University, D-79106 Freiburg, Germany
G. B. Stark
Affiliation:
Department of Plastic and Handsurgery, Valley Tissue Engineering Center, Albert-Ludwigs-University, D-79106 Freiburg, Germany
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Abstract

Primary human osteoblasts were cultered on biomaterials to develop living bone substitutes by Tissue Engineering. Monolayer cultures were established from iliac crest biopsies. Single cell suspensions were then seeded onto tricalciumphosphate (TCP) BIOBASE®, hydroxyapatitecollagen(HAC), autoclaved cancellous bone (CB) and fibrin glue (FG) Tissucol® The constructs were analyzed by electronmicroscopy, Cell proliferation assay (XTT) and histology to assess adhesion, proliferation and extracellular matrix formation on the biomaterial.

The osteoblastic cells survived in the fibrin glue and established a mesenchymal phenotype. They adhered to the HAC-sponges, TCP and CB. Prolifertion was seen on TCP and CB. HAC had a suppressive effect on the cells. An inmature matrix was found on TCP and a bone neomatrix on CB after 4 weeks in vitro.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 530: Symposium CC – Biomaterials Regulating Cell Function & Tissue Development , 1998 , 105
Copyright
Copyright © Materials Research Society 1998

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