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Potential Dependent Endothelial Cell Adhesion, Growth and Cytoskeletal Rearrangements

Published online by Cambridge University Press:  15 February 2011

Tiean Zhou ,
Susan J. Braunhut ,
Diane Medeiros  and
Kenneth A. Marx
Tiean Zhou
Affiliation:
Center for Intelligent Biomaterials and The Departments of Chemistry
Susan J. Braunhut
Affiliation:
Biological Sciences, University of Massachusetts, Lowell, MA 01854
Diane Medeiros
Affiliation:
Biological Sciences, University of Massachusetts, Lowell, MA 01854
Kenneth A. Marx
Affiliation:
Center for Intelligent Biomaterials and The Departments of Chemistry
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Abstract

Normal endothelial cells (ECs), lining the blood vessels, are influenced by their interaction with the underlying potentially piezoelectric extracellular matrix (ECM). That this interaction may affect the EC metabolic state and functions in vivo prompted us to study the subsequent response of cultured ECs on indium-tin oxide (ITO) glass electrodes subjected to 1 hr of constant DC surface potential ranging from -0.3 to +0.6 V (vs. Ag/AgCl). We measured, relative to controls, cellular viability, growth rate and changes in actin microfilament organization in ECs over a subsequent 6 days in culture. The growth rate of ECs was stimulated by negative potential and inhibited by positive potential. Differences could be detected as early as three days post-potential. We also observed a potential dependent cellular shape change and actin microfilament rearrangement at positive potentials within four days of treatment. ECs changed in average cell surface area and assumed a polygonal cell shape in response to treatment. Using NBD-phalloidin stain for actin and fluorescence microscopy, microfilaments were observed to re-distribute to the periphery of the cell at positive potential, indicative of cellular stress.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 489: Symposium K – Materials Science of the Cell , 1997 , 211
Copyright
Copyright © Materials Research Society 1998

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