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Porous Scaffolds Consisting of Collagen, Chondroitin Sulfate, and Hydroxyapatite with Enhanced Biodegradable Resistance for Cartilage Regeneration

Published online by Cambridge University Press:  14 March 2011

H. Kaneda ,
T. Ikoma ,
T. Yoshioka ,
M. Nishi ,
R. Matsumoto ,
T. Uemura ,
J. S. Cross  and
J. Tanaka
H. Kaneda
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo, Japan
T. Ikoma
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo, Japan
T. Yoshioka
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo, Japan
M. Nishi
Affiliation:
Nanosystem Research Institute (NRI), National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan.
R. Matsumoto
Affiliation:
Nanosystem Research Institute (NRI), National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan.
T. Uemura
Affiliation:
Nanosystem Research Institute (NRI), National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan.
J. S. Cross
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo, Japan
J. Tanaka
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo, Japan
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Abstract

Porous scaffolds of alkaline-soluble collagen including nanocomposite particles of chondroitin sulfate and low crystalline hydroxyapatite for cartilage regeneration were fabricated by freeze-drying and thermal dehydration treatments; porous collagen scaffolds were also synthesized as a reference. The scaffolds were cross-linked using glutaraldehyde (GA) vapor treatment in order to enhance biodegradable resistance. Microstructural observation with scanning electron microscope indicated that the scaffolds with and without GA cross-linkage had open pores between 130 to 200 μm in diameter and well-interconnected pores of 10 to 30 μm even after cross-linkage. In vitro biodegradable resistance to collagenase was significantly enhanced by GA cross-linking of the scaffolds. All these results suggest that the GA cross-linked scaffolds consisting of collagen, chondroitin sulfate, and low crystalline hydroxyapatite have suitable microporous structures and long-term biochemical stability for cartilage tissue engineering.

Keywords

biomaterialcompositetissue
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1301: Symposium V/NN/OO/PP – Soft Matter, Biological Materials and Biomedical Materials—Synthesis, Characterization and Applications , 2011 , mrsf10-1301-oo07-06
Copyright
Copyright © Materials Research Society 2011

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References

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