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Polyethyleneimine Functionalized ZnO Quantum Dots and their Binding Interaction with Bovine Serum Albumin Protein

Published online by Cambridge University Press:  14 March 2011

Prachi Joshi ,
Soumyananda Chakraborti ,
Pinak Chakrabarti ,
Surinder P. Singh ,
Z. A. Ansari  and
Virendra Shanker
Prachi Joshi
Affiliation:
National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi-110012, India. Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi-110025, India.
Soumyananda Chakraborti
Affiliation:
Department of Biochemistry, Bose Institute, P-1/12 CIT Scheme VIIM, Kolkata-700054, India.
Pinak Chakrabarti*
Affiliation:
Department of Biochemistry, Bose Institute, P-1/12 CIT Scheme VIIM, Kolkata-700054, India.
Surinder P. Singh*
Affiliation:
National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi-110012, India. Engineering Sciences and Materials Department, University of Puerto-Rico, Mayaguez, PR0680, USA.
Z. A. Ansari
Affiliation:
Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi-110025, India.
Virendra Shanker
Affiliation:
National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi-110012, India.
*
*Address for correspondence: [email protected]; [email protected]
*Address for correspondence: [email protected]; [email protected]
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Abstract

Water stable fluorescent ZnO quantum dots (QDs) have been synthesized by wet chemical route in presence of hydrophilic capping agent polyethyleneimine (PEI) as stabilizing agent. The binding interaction of prepared ZnO QDs is studied with bovine serum albumin (BSA) protein. X-ray diffraction measurement reveals hexagonal wurtzite structure of as synthesized ZnO QDs with an average size 4-6 nm, determined using Scherer’s equation and confirmed by transmission electron microscopy (TEM). The ZnO/PEI QDs exhibit strong yellow-green emission centered at 555 nm (2.23 eV). The interaction between BSA and ZnO/PEI QDs has been studied by using spectroscopic and calorimetric methods. Static mode of tryptophan quenching in BSA by ZnO/PEI QDs indicates that a ground state complex formation is taking place between ZnO/PEI and BSA, where, the week interactions (hydrogen bonding and hydrophobic interaction) are contributing towards the stability of the complex.

Keywords

luminescencenanostructuresurface chemistry
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1316: Symposium QQ – Nanofunctional Materials, Nanostructures and Nanodevices for Biomedical Applications II , 2011 , mrsf10-1316-qq03-07
Copyright
Copyright © Materials Research Society 2011

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Footnotes

$

Both authors have equal contribution

References

REFERENCES

1. Bruchez, M. Jr., Moronne, M., Gin, P., Weiss, S. and Alivisatos, A. P., Science 281, 2013–2016 (1998).Google Scholar
2. Larson, D. R., Zipfel, W. R., Williams, R. M., Clark, S. W., Bruchez, M. P., Wise, F. W. and Webb, W. W., Science 300, 1434–1436 (2003).Google Scholar
3. Wang, X., Kong, X., Yu, Y. and Zhang, H., J. Phys. Chem. C 111, 3836–3841 (2007).Google Scholar
4. Zhou, J., Xu, N. and Wang, Z. L., Adv. Mater. 18, 2432–2435 (2006).Google Scholar
5. Hanley, C., Layne, J., Punnoose, A., Reddy, K. M., Coombs, I., Coombs, A., Feris, K. and Wingett, D., Nanotechnology 19, 295103 (2008).Google Scholar
6. Moussodia, R. O., Balan, L. and Schneider, R., New J. Chem. 32, 1388–1393 (2008).Google Scholar
7. Wiseman, J. W, Goddard, C. A, McLelland, D. and H Colledge, W., Gene Ther. 10, 1654–1662 (2003).Google Scholar
8. Carrabino, S., Gioia, S. D., Copreni, E., Conese, E., J. Gene Med. 7, 1555–1564 (2005).Google Scholar
9. Jisha, V.S., Arun, K.T., Hariharan, M. and Ramaiah, D., J. Am. Chem. Soc. 128, 6024–6025 (2006).Google Scholar
10. Peters, T. Jr., Adv. Protein Chem. 37, 60065–60065 (1985).Google Scholar
11. Joshi, P., Ansari, Z. A., Singh, S. P. and Shanker, V., Adv. Sci. Lett. 2, 360–363 (2009).Google Scholar
12. Yadav, H. K., Sreenivas, K., Gupta, V., Singh, S. P., and Katiyar, R. S., J. Mater. Res. 22, 2404–2409 (2007).Google Scholar
13. Lakowicz, J.R., Principles of Fluorescence Spectroscopy. 3rd ed.: Springer: New York, 2006.Google Scholar
14. Ware, W.R., J. Phys. Chem. 66, 455–458 (1962).Google Scholar
15. Ross, P.D. and Subramanian, S., Biochemistry 20, 3096–3102 (1981).Google Scholar