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Polycaprolactone-Hydroxyapatite Composite Membrane Scaffolds for Bone Tissue Engineering

Published online by Cambridge University Press:  02 May 2013

Sabrina Morelli ,
Daniele Facciolo ,
Antonietta Messina ,
Antonella Piscioneri ,
Simona Salerno ,
Enrico Drioli  and
Loredana De Bartolo
Sabrina Morelli
Affiliation:
Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, Via P. Bucci, cubo 17/C, Rende (CS), Italy.
Daniele Facciolo
Affiliation:
Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, Via P. Bucci, cubo 17/C, Rende (CS), Italy.
Antonietta Messina
Affiliation:
Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, Via P. Bucci, cubo 17/C, Rende (CS), Italy. Department of Chemical Engineering and Materials, University of Calabria, Rende (CS)Italy.
Antonella Piscioneri
Affiliation:
Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, Via P. Bucci, cubo 17/C, Rende (CS), Italy.
Simona Salerno
Affiliation:
Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, Via P. Bucci, cubo 17/C, Rende (CS), Italy.
Enrico Drioli
Affiliation:
Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, Via P. Bucci, cubo 17/C, Rende (CS), Italy. Department of Chemical Engineering and Materials, University of Calabria, Rende (CS)Italy.
Loredana De Bartolo
Affiliation:
Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, Via P. Bucci, cubo 17/C, Rende (CS), Italy.
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Abstract

Bone tissue engineering typically involves the use of porous, bioresorbable scaffolds to serve as temporary, three-dimensional scaffolds to guide cell attachment, differentiation, proliferation, and subsequent tissue regeneration. In this study we developed a composite membrane scaffold by phase inversion technique by using biodegradable polyester, Polycaprolactone (PCL), with hydroxyapatite (HA) in order to develop novel controlled nanostructured biomaterials for bone tissue engineering applications.After preparation, membrane scaffolds were characterized in order to evaluate its morphological, physico-chemical and mechanical properties and then used for the cell culture.

Our experimental design consists to apply the knowledge of natural bone tissue remodelling in an in vitro membrane biohybrid system. We used human mesenchymal stem cells for culture in the membrane scaffolds inducing the differentiation in osteoblasts and human monocytes to trigger osteoclastogenesis. Osteoclastic resorption of the scaffold material would lead to subsequent induction of osteoblasts and faster bone formation with mesenchymal stem cells. Our results show that osteoblasts and osteoclasts were successfully differentiated in the developed PCL-HA membrane scaffold. This membrane system will lead to insights in the creation of a controllable osteoinductive microenvironment based on the specific properties (e.g. basic composition, surface chemistry, architecture) and on the function (resorption coupled to proliferation and differentiation) of defined cellular systems.

Keywords

adhesionbiomaterialcomposite
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1502: Symposium T – Membrane Material Platforms and Concepts for Energy, Environment and Medical Applications , 2013 , mrsf12-1502-t01-09
Copyright
Copyright © Materials Research Society 2013

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References

REFERENCES

Bose, S., Roy, M. and Bandyopadhyay, A., Trends in Biotechnology 30, 11 (2012).CrossRefGoogle Scholar
Zhao, F., Yin, Y.J., Lu, W.W., Leong, J.C., Zhang, W., Zhang, J.Y., Zhang, M.F. and Yao, K.D., Biomaterials 23, 3227 (2002).CrossRefGoogle Scholar
Vail, N.K., Swain, L.D., Fox, W.C., Aufdlemorte, T.B., Lee, G. and Barlow, J.W., Materials for biomedical application, Materials & Design 20, 123 (1999).Google Scholar
Kellomaki, M., Niiranen, H., Puumanen, K., Ashammakhi, N., Waris, T., and Törmälä, P.;, Biomaterials 21, 2495 (2000)CrossRefGoogle Scholar
Woodruff, M.A., Hutmacher, D.W., Progress in Polymer Science 35, 1217 (2010).CrossRefGoogle Scholar
Oh, S.H., Park, I.K., Kim, J.M., Lee, JH., Biomaterials 28, 1664 (2007).CrossRefGoogle Scholar
Guarino, V., Causa, F., Taddei, P., di Foggia, M., Ciapetti, G., Martini, D. et al. ., Biomaterials 29, 3662 (2008).CrossRefGoogle Scholar
Lam, CXF, Teoh, S.H., Hutmacher, D.W., Polym Int. 56, 718 (2007).CrossRefGoogle Scholar
Lam, CXF, Hutmacher, D.W., Schantz, J-T., Woodruff, M.A., Teoh, SH., J Biomed Mater Res Part A 90, 906 (2008).Google Scholar
Lam, CXF, Savalani, M.M., Teoh, S.H., Hutmacher, D., Biomed Mater; 3:1 (2008).CrossRefGoogle Scholar
Sawyer, A.A., Song, S.J., Susanto, E., Chuan, P., Lam, CXF, Woodruff, M.A. et al. ., Biomaterials 30, 2479 (2009).CrossRefGoogle Scholar
Hutmacher, D.W., Biomaterials 21, 2529 (2000).CrossRefGoogle Scholar
Abbah, S.A., Lam, CFX, Hutmacher, D.W., Goh, J.C.H., Wong, H-K, Biomaterials 30, 5086 (2009).CrossRefGoogle Scholar
Sopyana, I., Melb, M., Rameshc, S., Khalidd, K.A.. Science and Technology of Advanced Materials 8, 116 (2007).CrossRefGoogle Scholar
Ravichandran, R., Venugopal, J. R., Sundarrajan, S., Mukherjee, S. and Ramakrishna, S. Biomaterials 33, 846 (2012).CrossRefGoogle Scholar
Pittenger, M.F., Mackay, A.M., Beck, S.C., Jaiswal, R.K., Douglas, R., Mosca, J.D., Moorman, M.A., Simonetti, D.W., Craig, S., Marshak, D.R.. Science 284, 143 (1999).CrossRefGoogle Scholar
Gamie, Z., Tran, G.T., Vyzas, G., Korres, N., Heliots, M.., Mantalaris, A.., Tsiridis, E.. Expert Opin Biol Ther. 12, 713 (2012).CrossRefGoogle Scholar
Franceschi, R.T.. Crit Rev Oral Biol Med 10, 40 (1999).CrossRefGoogle Scholar
Chen, J., Singh, K., Mukherjee, B.B., Sodek, J.. Matrix 13, 113 (1993).CrossRefGoogle Scholar
Amoui, M., Suhr, S.M., Baylink, J.D. and William, K.H.. Am J Physiol Cell Physiol 287, C874 (2004).CrossRefGoogle Scholar
Minkin, C.. Calcif. Tissue Int. 34, 285–290 (1982).CrossRefGoogle Scholar