Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-06T05:01:39.402Z Has data issue: false hasContentIssue false
  • English
  • Français

A Photopaiterned Glucose Responsive Hydrogel for Use in a Conductimetric Sensor

Published online by Cambridge University Press:  15 February 2011

Matthew J. Lesho  and
Norman F. Sheppard Jr.
Matthew J. Lesho
Affiliation:
The Johns Hopkins University, Department of Biomedical Engineering 3400 Charles St., Baltimore, MD. 21218
Norman F. Sheppard Jr.
Affiliation:
The Johns Hopkins University, Department of Biomedical Engineering 3400 Charles St., Baltimore, MD. 21218
Get access

Abstract

The glucose responsive hydrogels developed by Horbett et al. [1, 2] are potentially useful as materials for constructing glucose sensors. The construction of microsensors presents challenges in developing processes for preparing these gels that are compatible with semiconductor fabrication technology.

We have lithographically patterned a pH-sensitive copolymer (poly-(2-hydroxyethyl methacrylate-co-dimethyl aminoethyl methacrylate) 90:10 (mole ratio) (HEMA/DMA) from a prepolymer solution of monomers, crosslinker, solvent, and a viscosity agent (p(HEMA)). Varying the spin speed and p(HEMA) concentration resulted in reproducible thicknesses between 5 and 25 μm. pH sensors produced by this method showed sensitivity ranging from 20 to 50%/pH and a response time (10%–90%) of 6.8 minutes. Sensitivity dropped by a factor of two over a 24 day testing period. The limited lifetime is thought to be due to loss of adhesion of the polymer to the substrate.

Derivatizing glucose oxidase with a photoactivatible crosslinker allowed it to be covalently immobilized onto the surface of the gel layer in a spatially specific manner. After immobilization, GOX specific staining showed that enzyme activity was preserved, however the amount of enzyme immobilized was insufficient to create a working glucose sensor.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 331: Symposium T – Biomaterials for Drug and Cell Delivery , 1993 , 193
Copyright
Copyright © Materials Research Society 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Albin, G. W., et al., J. Cont. Rel. 6, 267–291 (1987).Google Scholar
2. Kost, J., et al., J. Biomed. Mat. Res. 19, 1117–1133 (1985).Google Scholar
3. Siegel, R. A., et al., J. Contr. Rel. 11, 181–92 (1990).Google Scholar
4. McCurley, M. F., Seitz, W. R. in Biosensors and Chemical Sensors-Optimizing Performance Through Polymeric Materials, ed. Edelman, Peter and Wang, Joseph (ACS Symposium Series 487, Wash. D. C., 1992) pp. 301–309.Google Scholar
5. McCurley, M., Seitz, R., Analytca Chimica Acta 249, 373–380 (1991).Google Scholar
6. Hanazato, Y., et al., Analytica Chimica Acta 193, 87–96 (1987).Google Scholar
7. Hanazato, Y., et al., Analytica Chimica Acta 212, 49–59 (1988).Google Scholar
8. Sudholter, E. J. R., et al., 230, 59–65 (1990).Google Scholar
9. van den Berg, A., et al., Sens. Act. B 4, 235–38 (1991).Google Scholar
10. van den Berg, A., et al., Intl. Conf on Solid-State Sensors and Actuators, 1991, 233–236.Google Scholar
11. Cullen, D. C., et al., Anal. Chim. Acta 231(1), 33–40 (1990).Google Scholar
12. Pierce, Tech. report. #32060, 1989 Google Scholar
13. Lesho, M. J., Sheppard, N. F., Polym. Preprints 34, 850–851 (1993).Google Scholar
14. Siegel, R. A. in Resnonsive Gels: Volume Transitions I, ed. Dusek, K. (Advances in Polymer Science Berlin, 1993) pp. 231–267.Google Scholar
15. Michaeli, I., Katchalsky, A., J. Polym. Sci. 23, 603 (1957).Google Scholar