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A New Bio-Inorganic Nanocomposite Membrane for Glucose-Modulated Release of Insulin

Published online by Cambridge University Press:  31 January 2011

Claudia Regina Gordijo ,
Adam Jason Shuhendler  and
Xiao Yu Wu
Claudia Regina Gordijo
Affiliation:
[email protected], University of Toronto, Pharmaceutical Sciences, Toronto, Canada
Adam Jason Shuhendler
Affiliation:
[email protected], University Of Toronto, Pharmaceutical Sciences, 144 College St, Toronto, M5S3M2, Canada
Xiao Yu Wu
Affiliation:
[email protected], University of Toronto, Pharmaceutical Sciences, Toronto, Canada
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Abstract

This work focuses on the development of a new bio-inorganic nanocomposite glucose-responsive membrane to be applied as a single self-regulated platform for insulin delivery. Crosslinked bovine serum albumin (BSA)-based membranes were prepared containing impregnated pH-responsive poly(N-isopropyl acrylamide-co-methacrylic acid) nanoparticles (hydrogel NPs), glucose oxidase (GOx), catalase (CAT), with or without MnO2 NPs. The membrane acts as a glucose sensor and insulin release attenuator. In this system glucose is oxidized by GOx to produce gluconic acid, which regulates the permeability of the membrane to insulin. CAT and/or MnO2 NPs are introduced into the membrane in order to quench unwanted H2O2 produced by GOx turnover cycles, which can cause inactivation of GOx and toxicity. The glucose-modulated insulin release through the membrane is determined by alternating glucose concentration between 100 – 400 mg/dL (normal and hyperglycemic levels, respectively). The results show that the combination of CAT and MnO2 NPs in the membrane formulation leads to better efficiency in quenching the H2O2 and better long-term stability of GOx than using either alone. Very small amounts of insulin permeate though the membrane at the normal blood glucose level while a four-fold increase in the release rate is observed when glucose concentration is raised to a hyperglycemic level. The release rate of insulin drops when the glucose level is reduced to a normal value. These results demonstrate the self-regulated capability of the system.

Keywords

biomaterialnanostructurepolymer
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1234: Symposium QQ – Responsive Gels and Biopolymer Assemblies , 2009 , 1234-QQ03-02
Copyright
Copyright © Materials Research Society 2010

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References

1 Pickup, J. C., Zhi, Z. L., Khan, F., Saxl, T., Birch, D. J. S., Diabetes Metab. Res. Rev., 2008, 24, 604.Google Scholar
2 Parker, R. S., in Encyclopedia of Biomaterials and Biomedical Engineering, Vol. 1 (Eds: Wnek, G. E., Bowlin, G. L.), Informa Healthcare, USA, 2008, Ch. Insulin Deliver.Google Scholar
3 Ravaine, V., Ancla, C., Catargi, B., J. Controlled Release 2008, 132, 2.Google Scholar
4 Pepas, N. A., Bures, C. D., in Encyclopedia of Biomaterials and Biomedical Engineering, Vol. 1 (Eds: Wnek, G. E., Bowlin, G. L.), Informa Healthcare, USA, 2008, Ch. Glucose-Responsive Hydrogels.Google Scholar
5 Zhang, K., Wu, X. Y., J. Controlled Release 2002, 80, 169;Google Scholar
6 Zhang, K., Wu, X. Y., Biomaterials 2004, 25, 5281.Google Scholar
7 Huang, H.Y., Shaw, J., Yip, C., Wu, X.Y., Pharm. Res. 2008, 25, 1150.Google Scholar
8 Yam, F., Wu, X.Y., Zhang, Q., in Controlled Drug Delivery: Designing Technology for the Future, (Ed: Park, K.), ACS, Washington, DC, 2000, p. 263.Google Scholar
9 Wu, X.Y., Lee, P.I., Pharm. Res. 1993, 10, 1544.Google Scholar
10 Luo, X., Morrin, A., Killard, A. J., Smyth, M. R., Electroanalysis 2006, 4, 319.Google Scholar
11 Luo, X.-L., Xu, J.-J., Zhao, W., Chen, H.-Y., Biosens. Biolectron. 2004, 19, 1295.Google Scholar
12 Xu, J.-J., Chen, H.-Y., Electrochem. Commun. 2007, 9, 2611.Google Scholar
13 Fujimoto, T., Mizukoshi, Y., Nagata, Y., Maeda, Y., Oshima, R., Scr. Matter. 2001, 44, 2183.Google Scholar
14 Shin, M. K., Spinks, G. M., Shin, S. R., Kim, S. I., Kim, S. J., Adv. Mater. 2009, 21, 1.Google Scholar