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Nanopatterned Hydrogel Probes for Rapid Detection of Staphylococcus aureus

Published online by Cambridge University Press:  01 February 2011

Ishtiaq Saaem ,
Barry Kreiswirth  and
Matthew Libera
Ishtiaq Saaem
Affiliation:
Stevens Inst. of Technology, Dept of Chemical, Biomedical and Materials Engineering, Castle Point on Hudson. Stevens Institute of Technology, Hoboken, NJ 07030, Hoboken, NJ, 07030, United States
Barry Kreiswirth
Affiliation:
[email protected], Public Health Research Institute, Hospital Infections Program, Newark, NJ, 07103, United States
Matthew Libera
Affiliation:
[email protected], Stevens Inst. of Technology, Dept of Chemical, Biomedical and Materials Engineering, Hoboken, NJ, 07030, United States
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Abstract

We studied the use of nanopatterned hydrogels as a possible foundation for medical devices for the detection of Staphylococcus Aureus. Nanopatterned hydrogels, approximately 200 nm in diameter, were created by locally crosslinking dry amine-terminated poly(ethylene glycol) [PEG] (6000 Da) thin films using a focused electron beam. These gels then had a dry height of 50 nm and a swell ratio of about five. They were patterned into arrays with approximately 500 nm inter-gel spacing. These arrayed gels were functionalized with Immunoglobulin G [IgG], a biomolecule that binds Protein A. We are interested in seeing whether these arrayed gels can be used for bacterial capture and subsequent detection. We show, using an assay that binds IgG to Protein A expressing Staphylococcus Aureus [S.Aureus], that nanopatterned hydrogels can indeed be used as probes for detection. We further show that the specificity of our assay by using a knockout variant of S.Aureus that doesn't express Protein A as a negative control.

Keywords

biomedicalnanostructuredevices
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 954: Symposium H – Biofilm-Material Interactions — New Tools, Technologies and Opportunities , 2006 , 0954-H05-03
Copyright
Copyright © Materials Research Society 2007

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References

REFERENCES

1. Emori, T.G. and Gaynes, R.P., An overview of nosocomial infections, including the role of the microbiology laboratory. Clin Microbiol Rev. 6: p. 428–442.Google Scholar
2. Lowy, F.D., Staphylococcus aureus infections. N. Engl. J. Med, 1998(339): p. 520–532.Google Scholar
3. Koreen, L et al., spa typing method for discriminating among Staphylococcus aureus isolates: implications for use of a single marker to detect genetic micro- and macrovariation. J Clin Microbiol, 2004 Feb. 42(2): p. 792–9.Google Scholar
4. Krsko, P., et al., Electron-Beam Surface-Patterned Poly(ethylene glycol) Microhydrogels. Langmuir, 2003. 19(14): p. 5618–5625.Google Scholar
5. Hong, Y., Krsko, P., and Libera, M., Protein surface patterning using nanoscale PEG hydrogels. Langmuir, 2004 20(25): p. 11123–6.Google Scholar
6. Saaem, I., et al., Arrayed Nanohydrogels for Protein Array Technology. MRS Proceedings, 2005(0897-J06-07).Google Scholar
7. Neff, J., Tresco, P., and Caldwell, K., Surface modification for controlled studies of cellligand interactions. Biomaterials, 1999 20(23–24): p. 2377–93.Google Scholar
8. Higuchi, A, et al., Serum protein adsorption and platelet adhesion on pluronic-adsorbed polysulfone membranes. Biomaterials 2003. 24(19): p. 3235–45.Google Scholar
9. Shopsin, B, et al., Evaluation of protein A gene polymorphic region DNA sequencing for typing of Staphylococcus aureus strains. J Clin Microbiol, 1999 November. 37(11): p. 3556–63.Google Scholar