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Nano-fibrous scaffolding architecture enhances protein adsorption and cell attachment

Published online by Cambridge University Press:  11 February 2011

Kyung Mi Woo ,
Victor J. Chen  and
Peter X. Ma
Kyung Mi Woo
Affiliation:
Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, U.S.A.
Victor J. Chen
Affiliation:
Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, U.S.A.
Peter X. Ma
Affiliation:
Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, U.S.A.
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Abstract

Tissue engineering aims at resolving problems such as donor shortage and immune rejection faced in transplantation. Scaffolds (artificial extracellular matrices) play critical roles in tissue engineering. Recently, we developed nano-fibrous poly(L-lactic acid) (PLLA) scaffolds under the hypothesis that synthetic nano-fibrous scaffolding, mimicking the structure of natural collagen fibers, could create a more favorable microenvironment for cells. This is the first report that the nano-fibrous architecture built in three-dimensional scaffolds improved the features of protein adsorption, which mediates cell interactions with scaffolds. Scaffolds with nano-fibrous pore walls adsorbed 4 times more serum proteins than scaffolds with solid pore walls. More interestingly, the nano-fibrous architecture selectively enhanced protein adsorption including fibronectin and vitronectin, even though both scaffolds were made from the same PLLA material. Furthermore, nano-fibrous scaffolds also allowed more than 1.7 times of osteoblastic cell attachment than scaffolds with “solid” pore walls. These results demonstrate that the biomimetic nano-fibrous architecture serves as superior scaffolding for tissue engineering.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 735: Symposium C – Bioinspired Nanoscale Hybrid Systems , 2002 , C5.2
Copyright
Copyright © Materials Research Society 2003

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References

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