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Mucin Coating on Hydrophobic Polymer Materials

Published online by Cambridge University Press:  10 February 2011

L. Shi  and
K. D. Caldwell
L. Shi
Affiliation:
Center for Biopolymers at Interfaces, University of Utah, Salt Lake City, UT 84112
K. D. Caldwell
Affiliation:
Center for Biopolymers at Interfaces, University of Utah, Salt Lake City, UT 84112
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Abstract

In this work, the adsorption isotherm and kinetics of bovine submaxillary gland mucin (BSM) onto a hydrophobic polystyrene surface were studied by the solution depletion method, in which mucin surface concentrations were analyzed by amino acid analysis. Using a Langmuir adsorption model and non-linear curve fitting, kinetics parameters, kon and koff were determined. The coating was found to be very stable with very limited desorption (less than 2%) from a long term observation. By measuring the water contact angles, the changes in surface hydrophobicity due to mucin coating were monitored on four polymer materials, namely polymethylmethacrylate (PMMA), polyurethane (PU), polystyrene (PS), and silicone. After coating, all the hydrophobic surfaces turned into very hydrophilic. A strict correlation between mucin surface concentration and surface wettability has been found.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 599: Symposium DD – Mineralization in Natural & Synthetic Biomaterials , 1999 , 299
Copyright
Copyright © Materials Research Society 2000

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