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Microfluidic Cell Volume Biosensor for High Throughput Drug Screening

Published online by Cambridge University Press:  01 February 2011

Daniel A. Ateya ,
Frederick Sachs  and
Susan Z. Hua
Daniel A. Ateya
Affiliation:
Bio-MEMS and Bio-Materials Laboratory, Department of Mechanical and Aerospace Engineering, SUNY-Buffalo, Buffalo, NY 14260, USA
Frederick Sachs
Affiliation:
Hughes Center for Single Molecule Biophysics, Department of Physiology and Biophysics, SUNY-Buffalo, Buffalo, NY 14214, USA
Susan Z. Hua*
Affiliation:
Bio-MEMS and Bio-Materials Laboratory, Department of Mechanical and Aerospace Engineering, SUNY-Buffalo, Buffalo, NY 14260, USA Hughes Center for Single Molecule Biophysics, Department of Physiology and Biophysics, SUNY-Buffalo, Buffalo, NY 14214, USA
*
* Corresponding author: S.Z.H., E-mail: [email protected]
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Abstract

The maintenance of cell volume is critical to health. Cell volume change reflects many biological and physiological processes. We have developed a lab-chip to measure cell volume change in real-time with high sensitivity and resolution, and applicable to both adherent and suspended cell populations. The volume change was detected by measuring the impedance of extra-cellular solution within a microfluidic chamber containing the cells. Using microfabrication to make precise chamber dimensions, volume change can be detected in response to an osmotic gradient <1mOsm. The sensor provides rapid screening of pharmaceutical agents affecting cell volume. We have screened for peptides that affect cell volume regulation and found one in spider venom that inhibits at ∼100pM.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 845: Symposium AA – Nanoscale Materials Science in Biology and Medicine , 2004 , AA5.12
Copyright
Copyright © Materials Research Society 2005

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References

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