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MgO Nanomaterials Improve Fibroblast Adhesion and Proliferation

Published online by Cambridge University Press:  11 May 2015

Daniel J. Hickey  and
Thomas J. Webster
Daniel J. Hickey
Affiliation:
Department of Chemical Engineering, Northeastern University, Boston, MA 02115, U.S.A.
Thomas J. Webster
Affiliation:
Department of Chemical Engineering, Northeastern University, Boston, MA 02115, U.S.A. Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia
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Abstract

Magnesium (Mg) plays an important role in the body mediating cell-extracellular matrix (ECM) interactions, bone apatite structure and density, and nucleic acid chemistries. While Mg has been investigated as a biomaterial for bone applications, it has not been studied for applications within soft tissues. This study investigated, for the first time, the response of fibroblasts to magnesium oxide (MgO) nanoparticles for soft tissue engineering applications. Primary human dermal fibroblasts were cultured both on tissue culture polystyrene in media supplemented with MgO nanoparticles as well as on poly-L-lactic acid (PLLA)-MgO nanoparticle composites. As this study was conducted concurrently with a study aimed at bone tissue engineering, hydroxyapatite (HA) nanomaterials were used for comparison. Results showed for the first time that fibroblasts adhered onto MgO-containing composites roughly three times better than HA-PLLA samples and roughly 4.5 times better than plain PLLA samples. Fibroblasts also proliferated to statistically higher densities when cultured in medium supplemented with MgO nanoparticles compared to un-supplemented medium and medium supplemented with HA nanoparticles. These preliminary results together suggest that MgO nanoparticles should be further investigated as materials to improve the regeneration of soft tissues as well as bone.

Keywords

biomaterialMgnanoscale
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1722: Symposium F – Reverse Engineering of Bioinspired Nanomaterials , 2015 , mrsf14-1722-f05-13
Copyright
Copyright © Materials Research Society 2015 

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References

REFERENCES

Smith, L, Thomopoulos S. Tendon/Ligament-to-Bone Tissue Engineering - Current and Emerging Strategies. US Musculoskeletal Review 2011;6:11–5.Google Scholar
Maguire, ME, Cowan, JA. Magnesium chemistry and biochemistry. Biometals 2002;15(3):2013–210.Google ScholarPubMed
Webster, PO. Magnesium. American Journal of Clinical Nutrition 1987;45:1305–1312.CrossRefGoogle Scholar
Bigi, A, Foresti, E, Gregorini, R, Ripamonti, A, Roveri, N, Shah, JS. The role of magnesium on the structure of biological apatites. Calcif. Tissue Int. 1992;50:439–444.CrossRefGoogle ScholarPubMed
Tsuboi, S, Nakagaki, H, Ishiguro, K, Kondo, K, Mukai, M, Robinson, C, Weatherell, JA. Magnesium distribution in human bone. Calcif. Tissue Int. 1992;50:34–37.Google Scholar
Serre, CM, Papillard, M, Chavassieux, P, Voegel, JC, Boivin, G. Influence of magnesium substitution on a collagen-apatite biomaterial on the production of a calcifying matrix by human osteoblasts. J Biomed Mater Res. 1998;42:626–633.3.0.CO;2-S>CrossRefGoogle ScholarPubMed
Zreiqat, H, Howlett, CR, Zannettino, A, Evans, P, Schulze-Tanzil, G, Knabe, C, Shakibaei, M. Mechanisms of magnesium-stimulated adhesion of osteoblastic cells to commonly used orthopaedic implants. Journal of Biomedical Materials Research 2002;62:175–184.CrossRefGoogle ScholarPubMed
Yamniuk, AP, Vogel, HJ. Calcium- and magnesium-dependent interactions between calcium- and integrin-binding protein and the integrin αIIb cytoplasmic domain. Protein Science 2005;14(6):1429–1437.CrossRefGoogle ScholarPubMed
Gupta, B, Revagade, N, Hilborn, J. Poly(lactic acid) fiber: An overview. Progress in Polymer Science 2007;32:455–82.CrossRefGoogle Scholar
Li, W, Cooper, J Jr, Mauck, RL, Tuan, RS. Fabrication and characterization of six electrospun poly(alpha-hydroxy ester)-based fibrous scaffolds for tissue engineering applications. Acta Biomaterialia 2006;2:377–85.CrossRefGoogle ScholarPubMed
Sui, G, Yang, X, Mei, F, Hu, X, Chen, G, Deng, X, Ryu, S. Poly-L-lactic acid/hydroxyapatite hybrid membrane for bone tissue regeneration. J Biomed Mater Res A 2007;82:445–54.CrossRefGoogle ScholarPubMed
Liu, H, Webster, TJ. Mechanical properties of dispersed ceramic nanoparticles in polymer composites for orthopedic applications. International Journal of Nanomedicine 2010;2010:299–313.Google Scholar
Lee, JB, Park, HN, Ko, WK, Bae, MS, Heo, DN, Yang, DH, Kwon, IK. Poly(L-lactic acid)/hydroxyapatite nanocylinders as nanofibrous structure for bone tissue engineering scaffolds. J Biomed Nanotechnol 2013;9:424–29.CrossRefGoogle ScholarPubMed
Denkena, B, Lucas, A, Thorey, F, Waizy, H, Angrisani, N, Meyer-Lindenberg, A. Biocompatible magnesium alloys as degradable implant materials - Machining induced surface and subsurface properties and implant performance. Special Issues on Magnesium Alloys 2011.CrossRefGoogle Scholar
Weng, L, Webster, TJ. Nanostructured magnesium increases bone cell density. Nanotechnology 2012;23(48).CrossRefGoogle ScholarPubMed
Lopez-Macipe, A, Rodriguez-Clemente, R, Hidalgo-Lopez, A, Arita, I, Garcia-Garduno, MV, Rivera, E, Castano, VM. Wet chemical synthesis of hydroxyapatite particles from nonstoichiometric solutions. J Mater Synth Process 1998;6:21–26.CrossRefGoogle Scholar
Hickey, DJ, Webster, TJ. Adding MgO nanoparticles to hydroxyapatite-PLLA nanocomposites for improved bone tissue engineering applications. Submitted.Google Scholar