Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-19T05:10:33.446Z Has data issue: false hasContentIssue false
  • English
  • Français

Magnetoelastic Material as a Biosensor for the Detection of Salmonella Typhimurium

Published online by Cambridge University Press:  01 February 2011

Ramji S Lakshmanan ,
Rajesh Guntupalli ,
S. Huang ,
M. L. Johnson ,
Leslie C Mathison ,
I-Husan Chen ,
V. A. Petrenko ,
Zhong-Yang Cheng  and
B. A. Chin
Ramji S Lakshmanan
Affiliation:
[email protected], Auburn University, Materials Engineering, Auburn, Alabama, United States
Rajesh Guntupalli
Affiliation:
[email protected], Auburn University, Materials Engineering, Auburn, Alabama, United States
S. Huang
Affiliation:
[email protected], Auburn University, Materials Engineering, Auburn, Alabama, United States
M. L. Johnson
Affiliation:
[email protected], United States
Leslie C Mathison
Affiliation:
[email protected], Auburn University, Materials Engineering, Auburn, Alabama, United States
I-Husan Chen
Affiliation:
Auburn universityDepartment of Pathobiology, Auburn, Alabama
V. A. Petrenko
Affiliation:
[email protected], Auburn University, Pathobiology, Auburn, Alabama, United States
Zhong-Yang Cheng
Affiliation:
[email protected], Auburn University, Materials Engineering, Auburn, Alabama, United States
B. A. Chin
Affiliation:
[email protected], United States
Get access

Abstract

ABSTRACT Magnetoelastic materials are amorphous, ferromagnetic alloys that usually include a combination of iron, nickel, molybdenum and boron. Magnetoelastic biosensors are mass sensitive devices comprised of a magnetoelastic material that serves as the transducer and bacteriophage as the bio-recognition element. By applying a time varying magnetic field, the magnetoelastic sensor thin films can be made to oscillate, with the fundamental resonant frequency of oscillations depends on the physical dimensions and properties of the material. The change in the resonance frequency of these mass based sensors can be used to evaluate the amount of analyte attached on the sensor surface. Filamentous bacteriophage specific to S. typhimurium was used as a bio-recognition element in order to ensure specific and selective binding of bacteria onto the sensor surface. The sensitivity of magnetoelastic materials is known to be dependent on the physical dimensions of the material. An increase in sensitivity from 159Hz/decade for a 2mm sensor to 770Hz/decade for a 1mm sensor and 1100Hz/decade for a 500micron sensor was observed. The sensors were characterized by scanning electron microscopy (SEM) analysis assayed biosensors to provide visual verification of frequency responses and an insight into the characteristics of the distribution of phage on the sensor surface. The magnetoelastic sensors immobilized with filamentous phage are suitable for specific and selective detection of target analyte in different media. Certain modifications to the measurement circuit resulted in better signal to noise ratios for sensors with smaller dimensions (L<1mm). This was achieved by tuning the circuit resonance close to that of the sensor. According to models and preliminary tests, this method was anticipated in about a 5 times increase in signals for a 200×40×6microns. This technique and further studies into the design and modification of the measurement circuits could yield better, sensitive responses for sensors with smaller dimensions. The magnetoelastic materials offer further advantages of potential miniaturization, contact-less nature and ease of operation.

Keywords

actuatorsensorscanning electron microscopy (SEM)
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1129: Symposium V – Materials and Devices for Smart Systems III , 2008 , 1129-V02-04
Copyright
Copyright © Materials Research Society 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Mead, P.S., Slutsker, L., Dietz, V., McCaig, L.F., Bresee, J.S., Shapiro, C., Griffin, P.M., and Tauxe, R.V., Food-Related Illness and Death in the United States. Emerging Infectious Diseases, 1999. 5(5): 607–625.Google Scholar
2. Todd, E.C., Costs of acute bacterial foodborne disease in Canada and the United States. International Journal of Food Microbiology, 1989. 9(313–326).Google Scholar
3. Stoyanov, P.G. and Grimes, C.A., A Remote Query Magnetostrictive Viscosity Sensor. Sensors and Actuators A, 2000. 80: 8–14.Google Scholar
4. Landau, L.D. and Lifshitz, E.M., Theory of Elasticity. 1986: Pergamon Google Scholar
5. Lakshmanan, R.S., Guntupalli, R., Hu, J., Kim, D.-J., Petrenko, V.A., Barbaree, J.M., and Chin, B.A., Phage immobilized magnetoelastic sensor for the detection of Salmonella typhimurium. Journal of Microbiological Methods, 2007. 71(1): 55–59.Google Scholar
6. Lakshmanan, R.S., Guntupalli, R., Hu, J., Petrenko, V.A., Barbaree, J.M., and Chin, B.A., Detection of Salmonella typhimurium in fat free milk using a phage immobilized magnetoelastic sensor. Sensors and Actuators B: Chemical, 2007. 126(2): 544–549.Google Scholar
7. Guntupalli, R., Lakshmanan, R.S., Johnson, M.L., Hu, J., Huang, T.S., Barbaree, J.M., Vodyanoy, V.J., and Chin, B.A., Magnetoelastic biosensor for the detection of Salmonella typhimurium in food products. Sensing and Instrumentation for Food Quality and Safety, 2007. 1(1): 3–10.Google Scholar
8. Guntupalli, R., Hu, J., Lakshmanan, R.S., Huang, T.S., Barbaree, J.M., and Chin, B.A., A magnetoelastic resonance biosensor immobilized with polyclonal antibody for the detection of Salmonella typhimurium. Biosensors and Bioelectronics, 2007. 22: 1474–1479.Google Scholar
9. Ruan, C., Zeng, K., Varghese, O.K., and Grimes, C.A., Magnetoelastic Immunosensors: Amplified Mass Immunosorbent Assay for Detection of Escherichia coli O157:H7. Anal. Chem., 2003. 75: 6494–6498.Google Scholar
10. Jain, M.K., Schmidt, S., Mungle, C., Loiselle, K., Grimes, C. A., Measurement of temperature and liquid viscosity using magneto-acoustic/magneto-optical sensors. IEEE. Trans. on Magnetics, 2001. 37(4): 2767–2769.Google Scholar
11. Shankar, K., Zeng, K., Ruan, C., and Grimes, C.A., Quantification of ricin concentrations in aqueous media. Sensors and Actuators B, 2005. 107: 640–648.Google Scholar