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Localizing GABA Receptors and Glutamate Transporters Using Conjugated Quantum Dots in Rat Cerebellum Slices

Published online by Cambridge University Press:  31 January 2011

Abdel Illah El Abed ,
Anne Baudot ,
Mireille Chat ,
Sanaa Ben Khalifa  and
Gérard Louis
Abdel Illah El Abed
Affiliation:
[email protected], Univ. Paris Descartes, LNPC, Paris, France
Anne Baudot
Affiliation:
[email protected], Univ. Paris Descartes, LNPC, Paris, France
Mireille Chat
Affiliation:
[email protected], Univ. Paris Descartes, Lab. Physiologie Cérébrale, Paris, France
Sanaa Ben Khalifa
Affiliation:
[email protected], Univ. Paris Descartes, LNPC, Paris, France
Gérard Louis
Affiliation:
[email protected], Univ. Paris Descartes, LNPC, Paris, France
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Abstract

GABA and glutamate are known as the principal inhibitory and excitatory neurotransmitters in the vertebrate central nervous system, respectively. However, recent electro-physiological and immunogold data reported by Stell et al. [1] indicate that GABA may undergo also an excitatory action on presynaptic varicosities of parallel fibers (PFs) in the molecular layer of the rat cerebellum. PFs are axonal extensions, with a cross section of about 0.1 m, of the glutamatergic granule cells. Such an unexpected excitatory action of GABA indicates clearly the presence of GABA receptors in the PFs of granule cells. We show in this study that quantum dots may be used specifically and efficiently to label two endogenous synaptic proteins, namely R-GABAA-1 receptors (GABAA Rs) and glutamate transporters (VGLUT1) in order to target their localization in very small structures such as the presynaptic varicosities of the PFs, in agreement with the results recently reported by Stell et al..

Keywords

biologicaltissueluminescence
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1241: Symposium XX – Biological Imaging and Sensing Using Nanoparticle Assemblies , 2009 , 1241-XX03-07
Copyright
Copyright © Materials Research Society 2010

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