Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-27T20:25:19.767Z Has data issue: false hasContentIssue false
  • English
  • Français

Lipid Exchange Rates of Conventional and Polymer Stabilized Liposomes

Published online by Cambridge University Press:  01 February 2011

Awad Ahmed ,
Nicole Heldt ,
Gregory Slack  and
Yuzhuo Li
Awad Ahmed
Affiliation:
Department of Chemistry and Center for Advanced Materials Processing, Clarkson University, Potsdam, New York, 13699-5810, USA
Nicole Heldt
Affiliation:
Department of Chemistry and Center for Advanced Materials Processing, Clarkson University, Potsdam, New York, 13699-5810, USA
Gregory Slack
Affiliation:
Department of Chemistry and Center for Advanced Materials Processing, Clarkson University, Potsdam, New York, 13699-5810, USA
Yuzhuo Li
Affiliation:
Department of Chemistry and Center for Advanced Materials Processing, Clarkson University, Potsdam, New York, 13699-5810, USA
Get access

Abstract

Polymer-stabilized liposome systems consisting of polyethylene glycol bound lipids (PEG-lipids) and conventional (nonpolymer stabilized) liposomes were compared in terms of their inter-membrane lipid migration rates. In order to monitor the exchange of lipids between the membranes, 1-hexadecanoyl-2-(1-pyrenedecanoyl)-sn-glycero-3-phosphocholine (PY-PC), a phospholipid with pyrene attached to the hydrophobic tail, was used to label the liposome. Labeled and unlabeled liposome systems were mixed and fluorescence spectroscopy was used to examine the lipid transfer. More specifically, the relative employed to deduce the exchange kinetics. After labeled and unlabeled liposome systems were mixed, the E/M ratio for PY-PC in a polymer stabilized liposome system decreased by 66% over a period of 80 minutes, while the E/M for PY-PC in a conventional liposome system decreased 70% in less than 2 minutes. This suggests that the exchange rate for lipids in polymer stabilized liposome systems is much slower than that of conventional liposome systems. In addition, the exchange rates for both conventional and polymer stabilized liposome systems are accelerated at an elevated temperature.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 724: Symposium N – Biological and Biomimetic Materials Properties to Function , 2002 , N8.2
Copyright
Copyright © Materials Research Society 2002

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Lasic, D.D., Liposomes: form Physics to Applications, Elsevier, New York, 1993.Google Scholar
2. Woodle, M.C., Lasic, D.D., Biochim. Biophys. Acta., 1113, 171 (1992)Google Scholar
3. Nakajima, A., Bull. Chem. Soc. Japan, 44, 3272 (1971)Google Scholar
4. Almagren, M., Chem. Phys. Lett., 71, 539 (1980)Google Scholar
5. Doody, M.C., Pownall, H.J., Kao, Y.J., and Smith, L.C., Biochemistry, 19, 108 (1980)Google Scholar
6. Sengupta, P., Sackmann, E., Kuhnle, W., and Scholz, H.P., Biochim, Biophys. Acta 436, no. 4, 869 (1976)Google Scholar
7. Wolkowicz, P.E., Pownall, J.H., Pauly, D.F., and McMillin-Wood, J.B., Biochemistry, 23, 6426 (1984)Google Scholar
8. Ollman, M., Schwarzmann, G., Sandhoff, K., and Galla, H., Biochemistry, 26, 5943 (1987)Google Scholar
9. Li, W., Xue, L., Mayer, L.D., and Bally, M.B., Biochim. Biophys. Acta., 1513, 193 (2001)Google Scholar
10. Corley, P., and Loughrey, H.C., Biochim. Biophys. Acta., 1195, 149 (1994)Google Scholar
11. Forster, T., Agnew. Chem., 8, 333 (1969)Google Scholar
12. Samerharju, P.J., Virtanen, J.A., Eklund, K.K., Vainio, P., and Kinnunen, P.K.J, Biochemistry, 24, 2773 (1985)Google Scholar
13. L'Heureux, G.P. and Fragata, M., J. Photochem. Photobio. B, 3, 53 (1989)Google Scholar
14. Heldt, N., Gauger, M., Zhao, J., Slack, G., Pietryka, J., Li, Y., Reactive and Functional Polymers, 48, 181 (2001)Google Scholar
15. Roseman, M.A., and Thompson, T.E., Biochemistry, 19, 439 (1980)Google Scholar
16. Galla, H., and Hartman, W., Chem. Phys. Lipids., 27, 199 (1980)Google Scholar
17. Duportail, G., and Lianos, P., “Fluorescent probing of vesicles using pyrene and pyrene derivatives derivatives,” in Rosoff, M. (Ed.), Vesicles, Marcel Dekker, New York. 1996, pp. 295–372.Google Scholar