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Ligand-Receptor Contact Interactions Using Self-Assembled Bilayer Lipid Membranes

Published online by Cambridge University Press:  16 February 2011

A. Leitmannova Ottova  and
H. Ti Tien
A. Leitmannova Ottova
Affiliation:
Membrane Biophysics Laboratory (Giltner Hall), Department of Physiology, Michigan State University, East Lansing, MI 48824, (USA)
H. Ti Tien
Affiliation:
Membrane Biophysics Laboratory (Giltner Hall), Department of Physiology, Michigan State University, East Lansing, MI 48824, (USA)
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Abstract

Biological membranes play a crucial role in signal transduction and information processing as well as in energy conversion. This is owing to the fact that most physiological activities involve some kind of lipid bilayer-based receptor-ligand contact interactions. There are many outstanding examples such as ion sensing, antigen-antibody binding, and ligand/voltage--gated channels, to name a few. One approach to study these interactions in vitro is facilitated by employing artificial bilayer lipid membranes (BLMs). We have focused the efforts on ion and/or molecular selectivity and specificity using newly available self-assembled BLMs on solid support (i.e., s-BLMs), whose enhanced stability greatly aids in research areas of membrane biochemistry, biophysics and cell biology as well as in biosensor designs and molecular devices development. In this paper, our current work along with the experiments carried out in close collaboration with others on s-BLMs will be presented.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 360 , 1994 , 339
Copyright
Copyright © Materials Research Society 1995

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References

[1]Tien, H. T., “Membrane bioenergetics as viewed from reconstitution experiments,” in Redox Chemistry and Interfacial Behavior of Biological Molecules, Dryhurst, G. and Niki, K., eds., Plenum Publishing Corp., New York, NY, 1988. pp. 529–556.Google Scholar
[2]Tien, H. T., in Mittal, M. L. (ed), Proc. Sixth International Symp. on Surfactants in Solution, Vol. 8, Plenum, NY (1989). pp. 133-178Google Scholar
[3]Tien, H. T., “Electronic Processes and Redox Reactions in BLMs”, in Adv. Chem. Series, 235 Biomembrane Electrochemistry (Blank, M., ed.) ACS, Washington, DC, 1994. Chapter 24Google Scholar
[4]Tien, H. T., “Self-assembled lipid bilayers for biosensors and molecular electronic devices,” Advanced Materials, 2, 316–318, June 1990.Google Scholar
[5]Mountz, J. M. and Tien, H. T., “Photoeffects of pigmented BLMs in a microporous filter”, Photochem.Photobiol., 28, 395–400 (1978).Google Scholar
[6]Zviman, M. and Tien, H. T., Bioelectrochem. Bioenerg., in press (1994)Google Scholar
[7]Ottova-Leitmannova, A. and Tien, H. T., Prog. Surface Science, 41 4337–445 (1992).Google Scholar
[8]Hianik, T., Dlugopolsky, J. and Gyepessova, M., Bioelectrochem. Bioeng., 31, 99 (1993).Google Scholar
[9]Schulmann, W., Heyn, S.-P. and Gaub, H. E., Adv. Materials, 3, 388 (1991).Google Scholar
[10]Kinnear, K. T. and Monbouquette, H. G., Langmuir, 9, 2255 (1993).Google Scholar
[11]Ford, W. F. and Rodgers, M. A. J., J. Phys. Chem. 98 (1994) 7415Google Scholar
[12]Ottova-Leitmannova, A., Martynski, T., Wardak, A. and Tien, H. T., Abstracts, USARO Workshop, Cashiers, April 26-29, 1992. (Edited, Campbell, R.J. and Tove, S.R.). p. 54; in Molecular and Biomolecular Electronics (R. R. Birge, ed.) Adv. Chem. Series, No, 240, ACS, Washington, DC. 1994. pp. 439-454.Google Scholar
[13]O'Boyle, K., Siddiqi, F. A., and Tien, H. T., “Antigen-antibody-complement reaction studies on micro BLMs,” Immunol. Commun., 13, 85 (1984).Google Scholar
[14]Tien, H. T., Salamon, Z., Liu, W. and Ottova, A., Analyt. Lett., 26, 819 (1993).Google Scholar
[15]Kotowski, J., Janas, T., and Tien, H. T., “Immobilization of glucose oxidase on a polypyrrolelecithin bilayer lipid membrane,” J. Electroanal. Chem., 253, 277–282 (1988).Google Scholar
[16]Liu, W., Ottova-Leitmannova, A. and Tien, H. T., in “Polymer/Inorganic Interfaces”, Vol. 304 (Opila, R. L., Boerio, F. J. and Czanderna, A. W., eds) Materials Research Society, (1993).Google Scholar
[17]Ziegler, W., Remis, D., Brunovska, A. and Tien, H. T., in Proc. 7th C-S Conference on Thin Films, (Tvarozek, V. and Nemeth, S., Eds.) pp. 304–311.Google Scholar
[18]Tvarozek, V., Tien, H. T., Novotiny, I., Hianik, T., Dlugopolsky, J., Ziegler, W., Ottova, A. L., Jakabovic, J., Rehacek, V. and Uhlar, M., Snenors & Actuators: B. Chemical, 19, 597 (1994).Google Scholar
[19]Wang, L. G., Li, Y.-H. and Tien, H. T., Bioelectrochem. Bioenerg. in press (1994).Google Scholar
[20]Gu, L., Wang, L., Ottova, A. and Tien, H. T., Bioelectrochem. Bioenerg., submitted (1994).Google Scholar
[21]He, Y.-E., Xie, M.-G., Liu, S.-K., Ottova, A. L. and Tien, H. T., Anal. Lett., submitted (1994).Google Scholar
[22]Tai, Z.-H., Wang, D., Sun, X.-Q., Chem. J. Chinese Univ., 10, 760 (1989).Google Scholar
[23]Leitmannova-Ottova, A., W., , , Liu, Zhou, T.-A. and Tien, H. T., in Biomolecular Materials by Design, Alper, M., Bayley, H., Kaplan, D. and Navia, M., eds., MRS Symp. Proc. Series, Vol. 330, 1994.Google Scholar
[24]Leitmannova-Ottova, A., Guo, D.-L. and Tien, H. T., in Proc. of the 1994 N.A.Conference on Smart Structures and Materials, 13-18 Feb. 1994. Orlando, FLGoogle Scholar
[25]Tai, Z.-H., Li, T. and Yang, Z.-C., Prog. Biochem. Biophys., 19, 306, 391 (1992).Google Scholar
[26]Lu, X., Ottova, A. L. and Tien, H. T., Bioelectrochem. Bioenerg., submitted (1994)Google Scholar