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In Vitro Examination of Poly(glycerol sebacate) Degradation Kinetics: Effects of Porosity and Cure Temperature

Published online by Cambridge University Press:  04 February 2014

Nadia M. Krook ,
Courtney LeBlon  and
Sabrina S. Jedlicka
Nadia M. Krook
Affiliation:
Lehigh University Center for Advanced Materials & Nanotechnology, Bethlehem, PA, 18015, U.S.A
Courtney LeBlon
Affiliation:
Materials Science & Engineering, Center for Advanced Materials & Nanotechnology, Bethlehem, PA, 18015, U.S.A
Sabrina S. Jedlicka
Affiliation:
Lehigh University Center for Advanced Materials & Nanotechnology, Bethlehem, PA, 18015, U.S.A Mechanical Engineering & Mechanics, Center for Advanced Materials & Nanotechnology, Bethlehem, PA, 18015, U.S.A Bioengineering Program, Center for Advanced Materials & Nanotechnology, Bethlehem, PA, 18015, U.S.A
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Abstract

Poly(glycerol sebacate) (PGS) is a biodegradable and biocompatible elastomer that has been used in a wide range of biomedical applications. While a porous format is common for tissue engineering scaffolds, to allow cell ingrowth, PGS degradation has been primarily studied in a nonporous format. The purpose of this research was to investigate the degradation of porous PGS at three frequently used cure temperatures: 120°C, 140°C, and 165°C. The thermal, chemical, mechanical, and morphological changes were examined using thermogravimetric analysis, differential scanning calorimetry, Fourier transform infrared spectroscopy, compression testing, and scanning electron microscopy. Over the course of the 16-week degradation study, the samples’ pores collapsed. The specimens cured at 120°C demonstrated the most degradation and became gel-like after 16 weeks. Thermal changes were most evident in the 120°C and 140°C cure PGS specimens, as shifts in the melting and recrystallization temperatures occurred. Porous samples cured at all three temperatures displayed a decrease in compressive modulus after 16 weeks. This in vitro study helped to elucidate the effects of porosity and cure temperature on the biodegradation of PGS and will be valuable for the design of future PGS scaffolds.

Keywords

biomaterialpolymerscanning electron microscopy (SEM)
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1621: Symposium H/C/D/J – Advances in Structures, Properties and Applications of Biological and Bioinspired Materials , 2014 , pp. 87 - 92
Copyright
Copyright © Materials Research Society 2014 

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References

REFERENCES

Wang, Y, Kim, YM, Langer, R. (2002) In vivo degradation characteristics of poly(glycerol sebacate). J Biomed Mater Res 66A: 192–197.CrossRefGoogle Scholar
Wang, Y, et al. . (2002) A Tough Biodegradable Elastomer. Nature biotechnology 20.6: 602–606.CrossRefGoogle ScholarPubMed
Sun, Z, Chen, C, Sun, M, Ai, C, Lu, X, et al. . (2009) The application of poly (glycerol-sebacate) as biodegradable drug carrier. Biomaterials 30(28): 5209–5214.CrossRefGoogle ScholarPubMed
Motlagh, D, Yang, J, Lui, KY, Webb, AR, Ameer, GA. (2006) Hemocompatibility evaluation of poly(glycerol-sebacate) in vitro for vascular tissue engineering. Biomaterials 27(24): 4315–4324.CrossRefGoogle ScholarPubMed
Gao, J, Ensley, AE, Nerem, RM, Wang, Y. (2007) Poly(glycerol sebacate) supports the proliferation and phenotypic protein expression of primary baboon vascular cells. J Biomed Mater Res Part A 83A(4): 1070–1075.CrossRefGoogle Scholar
Gao, J, Crapo, P, Nerern, R, Wang, Y. (2008) Co-expression of elastin and collagen leads to highly compliant engineered blood vessels. J Biomed Mater Res Part A 85A(4): 1120–1128.10. Crapo, PM, Wang, Y. (2010) Physiologic compliance in engineered small-diameter arterial constructs based on an elastomeric substrate. Biomaterials 31(7): 1626–1635.CrossRefGoogle Scholar
Sales, VL, Engelmayr, GC Jr., Johnson, JA Jr., Gao, J, Wang, Y, et al. . (2007) Protein precoating of elastomeric tissue-engineering scaffolds increased cellularity, enhanced extracellular matrix protein production, and differentially regulated the Radisic, M, Park, H, Chen, F, Salazar-Lazzaro, JE, Wang, Y, et al. . (2006) Biomirnetic approach to cardiac tissue engineering: Oxygen carriers and channeled scaffolds. Tissue Eng 12(8): 2077–2091.Google Scholar
Radisic, M, Park, H, Gerecht, S, Cannizzaro, C, Langer, R, et al. . (2007) Biomimetic approach to cardiac tissue engineering. Philos Trans R Soc B-Biol Sci 362(1484): 1357–1368.CrossRefGoogle ScholarPubMed
Maidhof, R, Marsano, A, Lee, EJ, Vunjak-Novakovic, G. (2010) Perfusion seeding of channeled elastomeric scaffolds with myocytes and endothelial cells for cardiac tissue engineering. Biotechnol Prog 26(2): 565–572.Google ScholarPubMed
LeBlon, CE, Pai, R, Fodor, CR, Golding, AS, Coulter, JP, Jedlicka, SS. (2012). In vitro comparative biodegradation analysis of salt-leached porous polymer scaffolds.CrossRefGoogle Scholar
Kokubo, T, Kushitani, H, Sakka, S, Kitsugi, T, Yamamuro, T. (1990) Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W3 .CrossRefGoogle Scholar