Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-25T18:01:30.819Z Has data issue: false hasContentIssue false
  • English
  • Français

Impedance-based Biosensors

Published online by Cambridge University Press:  15 March 2011

X. Huang ,
D.W. Greve ,
I. Nausieda ,
D. Nguyen  and
M.M. Domach
X. Huang
Affiliation:
Department of Electrical and Computer Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, U.S.A
D.W. Greve
Affiliation:
Department of Electrical and Computer Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, U.S.A
I. Nausieda
Affiliation:
Department of Electrical and Computer Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, U.S.A
D. Nguyen
Affiliation:
Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, U.S.A
M.M. Domach
Affiliation:
Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, U.S.A
Get access

Abstract

Impedance measurements on arrays of microelectrodes can provide information about the growth, motility, and physiology of cells growing on the electrodes. In this talk, we report recent results obtained for the growth of 3T3 mouse fibroblasts and HCT116 human cancer cells on gold electrodes approximately 0.4 mm2 in area. Cells produce a characteristic peak in the impedance change plotted as a function of frequency. With the aid of electrical modeling of the cell-electrode system, the details of the changes in the measured impedance can be correlated to the cell size, fractional electrode coverage, and cell-electrode gap. In particular, comparison of impedance measurements of these two cell types show clear differences in the growth rate and the ratio of the cell-electrode gap to the cell size. In addition to presenting these experimental results illustrating the utility of electrode impedance measurements, we will outline the issues encountered when electrodes are scaled to cell size and incorporated into a matrix-addressed array.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 820: Symposia O/R – Nanoengineered Assemblies and Advanced Micro/Nanosystems , 2004 , O9.7
Copyright
Copyright © Materials Research Society 2004

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Giaever, I. and Keese, C.R., IEEE Transactions on Biomedical Engineering BME–33, 242–247 (1986).Google Scholar
2. Giaever, I. and Keese, C.R., Proc. Natl. Acad. Sci., 88, 7896–7900 (1991).Google Scholar
3. Huang, X., Nguyen, D., Greve, D.W., and Domach, M.M., (to be published in IEEE Sensors).Google Scholar
4. Huang, X., Greve, D.W., Nguyen, D., and Domach, M.M., in Proc. IEEE Int. Conf. Sensors 2003, pp. 305–309 (IEEE, Piscataway, NJ, 2004).Google Scholar
5. Nguyen, D.D., Huang, X., Greve, D.W., and Domach, M. M., (Biotechnology and Bioengineering, in press).Google Scholar
6. Partin, A.W., Isaacs, J.T., Treiger, B. and Coffey, D.S., Cancer Research 48 6050–3 (1988).Google Scholar
7. Latimer, J.J., in Developing Technologies for Early Detection of Breast Cancer, Newman, L., ed., summary of the workshop Committee on the Early Detection of Breast Cancer, pp. 14–15, National Cancer Policy Board, Institute of Medicine, (Washington, DC, 2000).Google Scholar
8. Giaever, I. and Keese, C.R., “Cell substrate electrical impedance sensor with multiple electrode array,” US patent 5,187,096 (1993).Google Scholar